Nanosecond to microsecond protein dynamics probed by magnetic relaxation dispersion of buried water molecules

Journal of the American Chemical Society
Erik Persson, Bertil Halle

Abstract

Large-scale protein conformational motions on nanosecond-microsecond time scales are important for many biological processes, but remain largely unexplored because of methodological limitations. NMR relaxation methods can access these time scales if protein tumbling is prevented, but the isotropy required for high-resolution solution NMR is then lost. However, if the immobilized protein molecules are randomly oriented, the water 2H and 17O spins relax as in a solution of freely tumbling protein molecules, with the crucial difference that they now sample motions on all time scales up to approximately 100 micros. In particular, the exchange rates of internal water molecules can be determined directly from the 2H or 17O magnetic relaxation dispersion (MRD) profile. This possibility opens up a new window for characterizing the motions of individual internal water molecules as well as the large-scale protein conformational fluctuations that govern the exchange rates of structural water molecules. We introduce and validate this new NMR method by presenting and analyzing an extensive set of 2H and 17O MRD data from cross-linked gels of two model proteins: bovine pancreatic trypsin inhibitor and ubiquitin. We determine residence times ...Continue Reading

References

Sep 1, 1992·Journal of Biomolecular NMR·E LiepinshK Wüthrich
Apr 5, 1987·Journal of Molecular Biology·S Vijay-KumarW J Cook
Jul 1, 1995·Biophysical Journal·B Halle, V P Denisov
Aug 1, 1994·Protein Science : a Publication of the Protein Society·M A WilliamsJ M Thornton
Dec 1, 1993·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·S H Koenig, R D Brown
Jan 1, 1996·Faraday Discussions·V P Denisov, B Halle
Apr 26, 2001·Acta Crystallographica. Section D, Biological Crystallography·A AddlagattaM Jaskolski
May 23, 2002·Biophysical Journal·Patrizia Cioni, Giovanni B Strambini
May 29, 2003·Biophysical Journal·Michael GottschalkBertil Halle
Jan 8, 2004·Journal of the American Chemical Society·Kristofer ModigBertil Halle
Feb 5, 2004·Journal of the American Chemical Society·Jens Dittmer, Geoffrey Bodenhausen
Aug 13, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Bertil Halle
Feb 22, 2005·Protein Science : a Publication of the Protein Society·Francesca MassiArthur G Palmer
Mar 3, 2005·Journal of Molecular Biology·Ryo KitaharaKazuyuki Akasaka
Mar 7, 2006·Journal of Biomolecular NMR·Nils A LakomekJens Meiler
Apr 6, 2006·Journal of the American Chemical Society·Fabian Vaca ChavezBertil Halle
Apr 15, 2006·Science·Anthony Mittermaier, Lewis E Kay
May 11, 2006·Chemical Reviews·Stewart A Adcock, J Andrew McCammon
May 30, 2006·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Bertil Halle
Jul 21, 2006·The Journal of Physical Chemistry. B·Kei Moritsugu, Jeremy C Smith
Oct 13, 2006·The Biochemical Journal·James H HurleyGali Prag
Oct 27, 2006·The Journal of Physical Chemistry. B·Fabian Vaca ChavezBertil Halle
Dec 1, 2006·Physical Chemistry Chemical Physics : PCCP·Erika BalogDavid Perahia
Dec 13, 2006·Journal of Molecular Biology·W Miachel HansonDavid P Goldenberg
May 1, 2005·Journal of Chemical Theory and Computation·Aart J Nederveen, Alexandre M J J Bonvin

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Citations

Jan 5, 2011·Nature Structural & Molecular Biology·Nathaniel V NucciA Joshua Wand
Jul 30, 2010·Physical Chemistry Chemical Physics : PCCP·Kristofer ModigBertil Halle
Aug 18, 2010·Physical Chemistry Chemical Physics : PCCP·Marion JasninGiuseppe Zaccai
Apr 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Erik Persson, Bertil Halle
Nov 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Erik P SundeBertil Halle
Aug 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sayan GuptaMark R Chance
Jan 22, 2014·Physical Chemistry Chemical Physics : PCCP·Shuji KaiedaBertil Halle
Jun 21, 2011·The Journal of Chemical Physics·Jianhui Tian, Angel E García
Jan 22, 2013·Annual Review of Physical Chemistry·Chi-Yuan Cheng, Songi Han
Dec 17, 2014·The Journal of Chemical Physics·Elise Duboué-Dijon, Damien Laage
Oct 16, 2010·Science·David E ShawWilly Wriggers
Jul 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Filip Persson, Bertil Halle
Mar 26, 2011·Biophysical Chemistry·Kunitsugu SodaMakoto Taiji
Feb 19, 2010·Journal of Magnetic Resonance·Erik P Sunde, Bertil Halle
Jul 9, 2009·Protein Science : a Publication of the Protein Society·Carlos J LópezWayne L Hubbell
Jan 21, 2010·Biophysical Journal·Galina DiakovaRobert G Bryant
Apr 15, 2014·Biochimica Et Biophysica Acta·Håkan NilssonJohannes Messinger
Jul 22, 2009·Biophysical Journal·Martin LignellHans-Christian Becker
Apr 10, 2015·Magnetic Resonance in Chemistry : MRC·Rebecca M SteeleSalvatore Bubici
Jul 28, 2016·The Journal of Chemical Physics·Zhiwei Chang, Bertil Halle
Dec 16, 2016·Journal of the American Chemical Society·Monika StachuraLars Hemmingsen
Mar 5, 2017·Current Opinion in Chemical Biology·Michael J Fink, Per-Olof Syrén
Dec 4, 2009·Journal of the American Chemical Society·Erik P Sunde, Bertil Halle
Mar 5, 2016·Chemical Society Reviews·Cyril CharlierFabien Ferrage
Aug 6, 2016·Physical Chemistry Chemical Physics : PCCP·Vered Wineman-FisherYifat Miller
Jul 6, 2014·Nature Communications·Håkan NilssonJohannes Messinger
Oct 14, 2017·Nature Communications·Martijn TrosSander Woutersen
Nov 1, 2015·Biochemical Society Transactions·Vassiliy N BavroCorie Ralston
Feb 21, 2009·Faraday Discussions·Johan QvistBertil Halle
Jul 16, 2016·Angewandte Chemie·Samuel F CousinFabien Ferrage
Sep 1, 2016·Journal of Synchrotron Radiation·Sayan GuptaCorie Y Ralston
Jun 10, 2018·The Journal of Chemical Physics·Filip PerssonBertil Halle
Sep 3, 2017·The Journal of Chemical Physics·Zhiwei Chang, Bertil Halle
Mar 10, 2010·The Journal of Physical Chemistry. B·Yubo Fan, Yi Qin Gao
Apr 25, 2009·The Journal of Physical Chemistry. B·M Trivikram RaoV S S Sastry
Jan 1, 2009·Journal of the American Chemical Society·Monika DavidovicBertil Halle
Apr 25, 2021·Biophysical Journal·Yuji TokunagaMasahiko Imashimizu
Jul 6, 2010·The Journal of Physical Chemistry. B·Giovanni B Strambini, Margherita Gonnelli

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