Nanosomal Microassemblies for Highly Efficient and Safe Delivery of Therapeutic Enzymes

ACS Applied Materials & Interfaces
Huarong XiongJingqing Zhang

Abstract

Enzyme therapy has unique advantages over traditional chemotherapies for the treatment of hyperuricemia, but overcoming the delivery obstacles of therapeutic enzymes is still a significant challenge. Here, we report a novel and superior system to effectively and safely deliver therapeutic enzymes. Nanosomal microassemblies loaded with uricase (NSU-MAs) are assembled with many individual nanosomes. Each nanosome contains uricase within the alkaline environment, which is beneficial for its catalytic reactions and keeps the uricase separate from the bloodstream to retain its high activity. Compared to free uricase, NSU-MAs exhibited much higher catalytic activity under physiological conditions and when subjected to different temperatures, pH values and trypsin. NSU-MAs displayed increased circulation time, improved bioavailability, and enhanced uric acid-lowering efficacy, while decreasing the immunogenicity. We also described the possible favorable conformational changes occurring in NSU-MAs that result in favorable outcomes. Thus, nanosomal microassemblies could serve as a valuable tool in constructing delivery systems for therapeutic enzymes that treat various diseases.

References

May 15, 2003·Drug Delivery·B LuH Yang
Sep 29, 2009·International Journal of Pharmaceutics·Q Y TanJ Q Zhang
Aug 20, 2010·Journal of Pain & Palliative Care Pharmacotherapy·UNKNOWN U.S. Food And Drug Administration
Sep 25, 2010·Methods in Molecular Biology·Makoto Yoshimoto
Apr 3, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yan-Zi QiuFa-Jun Song
Jun 19, 2012·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Qunyou TanLiangke Zhang
Jun 23, 2012·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Chun ZhangFei Liao
Jul 31, 2012·Drugs of Today·R L George, J S Sundy
Dec 15, 2012·Biochemistry·Michael S CavesRobert B Freedman
May 21, 2013·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Maria A Lopez-OlivoAbdulla Salahudeen
Jul 11, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Christian GerathsWilfried Weber
Feb 21, 2014·High Blood Pressure & Cardiovascular Prevention : the Official Journal of the Italian Society of Hypertension·Davide GrassiClaudio Ferri
Mar 25, 2014·FEBS Letters·Nathalie Colloc'h, Thierry Prangé
Mar 29, 2014·Biotechnology and Applied Biochemistry·Ya HuangFei Liao
May 23, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Li-Min LiuTzong-Der Way
Jun 26, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Jiangbo HuJingqing Zhang
Jul 20, 2014·Pharmaceutical Research·M Luísa CorvoM Bárbara A F Martins
Jan 13, 2015·Advances in Colloid and Interface Science·A Molaei, K E Waters
Feb 11, 2015·Biochimica Et Biophysica Acta·Ashley N MartfeldRoger E Koeppe

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.