Nanostructural Analysis of Enzymatic and Non-enzymatic Brown Rot Fungal Deconstruction of the Lignocellulose Cell Wall†

Frontiers in Microbiology
Yuan ZhuBarry Goodell

Abstract

Brown rot (BR) decay mechanisms employ carbohydrate-active enzymes (CAZymes) as well as a unique non-enzymatic chelator-mediated Fenton (CMF) chemistry to deconstruct lignocellulosic materials. Unlike white rot fungi, BR fungi lack peroxidases for lignin deconstruction, and also lack some endoglucanase/cellobiohydrolase activities. The role that the CMF mechanism plays in "opening up" the wood cell wall structure in advance of enzymatic action, and any interaction between CMF constituents and the selective CAZyme suite that BRs possess, is still unclear. Expression patterns for CMF redox metabolites and lytic polysaccharide monooxygenase (LPMO-AA9 family) genes showed that some LPMO isozymes were upregulated with genes associated with CMF at early stages of brown rot by Gloeophyllum trabeum. In the structural studies, wood decayed by the G. trabeum was compared to CMF-treated wood, or CMF-treated wood followed by treatment with either the early-upregulated LPMO or a commercial CAZyme cocktail. Structural modification of decayed/treated wood was characterized using small angle neutron scattering. CMF treatment produced neutron scattering patterns similar to that of the BR decay indicating that both systems enlarged the nanopore ...Continue Reading

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Citations

Apr 19, 2021·Biotechnology for Biofuels·Fernanda Lopes de FigueiredoAndré Damasio
Jul 30, 2021·Frontiers in Microbiology·Stefanos StravoravdisBarry Goodell

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Datasets Mentioned

BETA
GSE108189

Methods Mentioned

BETA
small angle neutron scattering
RNA-seq
SANS
X-ray
neutron scattering
Enzyme Treatment
enzymatic treatments
electron microscopy
Small

Software Mentioned

IRENA
IgorPro
BlastP

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