Nanostructure-initiator mass spectrometry: a protocol for preparing and applying NIMS surfaces for high-sensitivity mass analysis

Nature Protocols
Hin-Koon WooGary Siuzdak

Abstract

Nanostructure-initiator mass spectrometry (NIMS) is a new surface-based MS technique that uses a nanostructured surface to trap liquid ('initiator') compounds. Analyte materials adsorbed onto this 'clathrate' surface are subsequently released by laser irradiation for mass analysis. In this protocol, we describe the preparation of NIMS surfaces capable of producing low background and high-sensitivity mass spectrometric measurement using the initiator compound BisF17. Examples of analytes that adsorb to this surface are small molecules, drugs, lipids, carbohydrates and peptides. Typically, NIMS is used to analyze samples ranging from simple analytical standards and proteolytic digests to more complex samples such as tissues, cells and biofluids. Critical experimental considerations of NIMS are described. Specifically, NIMS sensitivity is examined as a function of pre-etch cleaning treatment, etching current density, etching time, initiator composition, sample concentration, sample deposition method and laser fluence. Typically, NIMS surface preparation can be completed in less than 2 h. Subsequent sample preparation requires 1-5 min, depending on sample deposition method. Mass spectrometric data acquisition typically takes 1-30 s...Continue Reading

References

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Citations

Mar 13, 2012·Analytical and Bioanalytical Chemistry·Matthew GrevingTrent Northen
Nov 6, 2010·Analytical Chemistry·Matthew P GrevingGary Siuzdak
Nov 14, 2012·Analytical Chemistry·Jay G ForsytheJohn A McLean
Mar 24, 2009·Analytical Chemistry·Oscar YanesGary Siuzdak
Mar 23, 2012·Nature Reviews. Molecular Cell Biology·Gary J PattiGary Siuzdak
May 1, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Do Yup LeeTrent Northen
Nov 19, 2010·Bioanalysis·Richard J A Goodwin, Andrew R Pitt
Nov 19, 2010·Bioanalysis·Jun HanChristoph H Borchers
Jul 23, 2013·Bioinformatics·Theodore Alexandrov, Andreas Bartels
Apr 10, 2013·Analytical and Bioanalytical Chemistry·Tristan de RondJay D Keasling
May 29, 2014·Journal of Pharmaceutical and Biomedical Analysis·Tadashi HayamaHitoshi Nohta
Jan 8, 2016·Analytical Chemistry·Jian GaoTrent R Northen
Mar 1, 2012·Journal of Proteomics·Daisuke MiuraHiroyuki Wariishi
Aug 19, 2014·Current Opinion in Biotechnology·Tristan de RondTrent Northen
Dec 23, 2014·Metabolomics : Official Journal of the Metabolomic Society·Gary J PattiGary Siuzdak
Apr 16, 2013·Scientific Reports·Katherine B LouieTrent R Northen
Jun 25, 2016·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Berin A BoughtonUte Roessner
Mar 31, 2015·Journal of the American Society for Mass Spectrometry·Mohammadreza ShariatgorjiPer E Andren
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Apr 30, 2013·Progress in Lipid Research·Shane R EllisTodd W Mitchell
May 12, 2012·The Biochemical Journal·Jay J Thelen, Ján A Miernyk
May 12, 2015·Cell Metabolism·Caroline H JohnsonGary Siuzdak
Dec 3, 2014·Biochimica Et Biophysica Acta·Chuanzi OuYangLingjun Li
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Sep 28, 2010·Drug Testing and Analysis·James Kerwin
Dec 16, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Ryuichi Arakawa, Hideya Kawasaki
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Apr 2, 2013·Analytical Methods : Advancing Methods and Applications·Robert M SturmLingjun Li
May 21, 2019·NMR in Biomedicine·Sean Philip ArlauckasEdward James Delikatny

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