Nanostructured poly(3,4-ethylenedioxythiophene)-metalloporphyrin films: improved catalytic detection of peroxynitrite

Biosensors & Bioelectronics
S F PeteuMekki Bayachou

Abstract

We investigated in this paper the sensing performance of inherently conductive polymer, poly(3,4-ethylenedioxythiophene) (PEDOT), functionalized with hemin (iron protoporphyrin) as an electrocatalytic reporter. The sensing platform is prepared by electrodeposition of a composite film of hemin-PEDOT on a 30-microm diameter carbon fiber electrode (CFE). The polymerized films were characterized by field emission scanning electron microscopy (FESEM), which pointed to nanostructured films with tortuous pores. The electrocatalytic oxidation of peroxynitrite was characterized by cyclic voltammetry as well as other electrochemical methods. The catalytic current is proportional to the analyte's concentration. Optimized hemin-PEDOT modified CFEs were utilized for the first time to detect ONO2(-), with a response time down to 5 s and a limit of detection as low as 200 nM as evidenced by amperometry. Our hemin-PEDOT modified CFEs have a sensitivity of 13 nA/microM, ca.130 times higher than the bare CFE. More work is underway using other metalloporphyrins as electrocalalysts to improve the detection limit, the selectivity, and to further miniaturize these hemin-PEDOT modified electrodes.

Citations

Sep 4, 2012·Analytical and Bioanalytical Chemistry·C ZanardiR Seeber
May 18, 2013·Analytica Chimica Acta·Serban F PeteuMekki Bayachou
Jun 5, 2013·The Analyst·Raluca OpreaSabine Szunerits
Jul 21, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wu LeiXifeng Xia
Apr 10, 2019·Polymers·Monika Tomczykowa, Marta Eliza Plonska-Brzezinska

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