Nanostructured Surfaces That Mimic the Vascular Endothelial Glycocalyx Reduce Blood Protein Adsorption and Prevent Fibrin Network Formation

ACS Applied Materials & Interfaces
Mohammadhasan HedayatiMatt J Kipper

Abstract

Blood-contacting materials are critical in many applications where long-term performance is desired. However, there are currently no engineered materials used in cardiovascular implants and devices that completely prevent clotting when in long-term contact with whole blood. The most common approach to developing next-generation blood-compatible materials is to design surface chemistries and structures that reduce or eliminate protein adsorption to prevent blood clotting. This work proposes a new paradigm for controlling protein-surface interactions by strategically mimicking key features of the glycocalyx lining the interior surfaces of blood vessels: negatively charged glycosaminoglycans organized into a polymer brush with nanoscale domains. The interactions of two important proteins from blood (albumin and fibrinogen) with these new glycocalyx mimics are revealed in detail using surface plasmon resonance and single-molecule microscopy. Surface plasmon resonance shows that these blood proteins interact reversibly with the glycocalyx mimics, but have no irreversible adsorption above the limit of detection. Single-molecule microscopy is used to compare albumin and fibrinogen interactions on surfaces with and without glycocalyx-m...Continue Reading

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Citations

Feb 26, 2020·Physical Chemistry Chemical Physics : PCCP·Mohammadhasan HedayatiDiego Krapf
Apr 7, 2020·The Journal of Trauma and Acute Care Surgery·Teryn R RobertsAndriy I Batchinsky
Jan 23, 2021·Journal of Colloid and Interface Science·Mohammadhasan HedayatiMatt J Kipper
May 16, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Paulo C F da CâmaraMatt J Kipper
Apr 30, 2021·International Journal of Biological Macromolecules·Késsily B RufatoAlessandro F Martins
Dec 17, 2021·Frontiers in Medicine·Barbara J BallermannBörje Haraldsson

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