Nanotechnology as a New Therapeutic Approach to Prevent the HIV-Infection of Treg Cells

PloS One
Didiana Jaramillo-RuizM Angeles Muñoz-Fernández

Abstract

HIV-1 has proved to infect regulatory T cells (Treg) modifying their phenotype and impairing their suppressive capacity. As Treg cells are a crucial component in the preservation of the immune homeostasis, we researched that the antiviral capacity of carboxilan dendrimers prevents the HIV-1 infection of Treg and their effects. The phenotype and suppressive capacity of Treg treated or non-treated with carbosilane dendrimers were studied by flow cytometry. Treated and non-treated Treg from healthy donors were infected with HIV-1NL4.3. The infection of Treg cells by HIV-1, and protective effect of two dendrimers were determined by measuring antigen p24gag in the supernatant of the culture and intracellular. The Treg cells were treated with cationic and anionic carbosilane dendrimers. The results showed that both dendrimers did not modify the phenotype and functionality of Treg cells compared with non- treated Treg cells. Anionic dendrimers showed high biocompatibility with normal activity of the Treg cells and in antiviral assays. These dendrimers were highly active against HIV-1 preventing the infection of Treg, and were able to protect the Treg from the Foxp3 downregulation induced by the HIV-1 infection. This is the first work ...Continue Reading

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Citations

Jun 22, 2017·Therapeutic Delivery·Blessing A Aderibigbe
May 17, 2019·Journal of Nanobiotechnology·Alba Martín-MorenoMª Angeles Muñoz-Fernández
Jun 10, 2020·Pharmaceutics·Carlos Guerrero-BeltránMaría Ángeles Muñoz-Fernández
Jul 28, 2017·Therapeutic Advances in Infectious Disease·Lavanya SinghRaveen Parboosing
Dec 17, 2020·International Journal of Molecular Sciences·Ignacio Relaño-Rodríguez, Maria Ángeles Muñoz-Fernández
Jan 14, 2021·Nanomaterials·John Jairo Aguilera-CorreaMaría Vallet-Regí
May 2, 2021·Microbial Pathogenesis·Muthupandian SaravananHamed Barabadi

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Methods Mentioned

BETA
flow cytometry
transfection
ELISA

Software Mentioned

GraphPad Prism

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