Nanotechnology Based Repositioning of an Anti-Viral Drug for Non-Small Cell Lung Cancer (NSCLC)

Pharmaceutical Research
Vineela ParvathaneniVivek Gupta

Abstract

Nelfinavir (NFV), a FDA approved antiretroviral drug, has been reported to exhibit cancer cells growth inhibition and increased apoptosis. However, it requires a higher dose leading to toxicity, thus limiting its potential clinical translation. We aim to develop biodegradable (poly (lactic-co-glycolic acid)) PLGA nanoparticles of nelfinavir and determine their efficacy to treat non-small cell lung cancer (NSCLC). HIV protease inhibitor, NFV, was loaded into PLGA nanoparticles by double emulsion/solvent evaporation method; and nanoparticles were characterized for physicochemical characteristics including morphology and intracellular uptake. Their anti-cancer efficacy in NSCLC was assessed by in vitro assays including cytotoxicity, cellular migration, colony formation; and 3D spheroid culture mimicking in-vivo tumor microenvironment. Studies were also conducted to elucidate effects on molecular pathways including apoptosis, autophagy, and endoplasmic stress. NFV loaded PLGA nanoparticles (NPs) were found to have particle size: 191.1 ± 10.0 nm, zeta potential: -24.3 ± 0.9 mV, % drug loading: 2.5 ± 0.0%; and entrapment efficiency (EE): 30.1 ± 0.5%. NFV NP inhibited proliferation of NSCLC cells compared to NFV and exhibited signific...Continue Reading

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Citations

Feb 14, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Nishant S KulkarniVivek Gupta
Sep 4, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Snehal K ShuklaVivek Gupta

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Methods Mentioned

BETA
xenografts
dynamic light scattering
transmission electron microscopy
Fluorescence Microscopy
Assay
Fluorescence
Protein Assay
Atomic force microscopy
AFM

Software Mentioned

GraphPad Prism
Open CFU
GraphPad
ImageJ

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