Nanotoxicity of Silver Nanoparticles on HEK293T Cells: A Combined Study Using Biomechanical and Biological Techniques

ACS Omega
Xuefeng JiangHuabin Wang

Abstract

Human embryonic kidney 293T cells (HEK293T cells) before and after treatment with silver nanoparticles (AgNPs) were measured using advanced atomic force microscopy (AFM) force measurement technique, and the biomechanical property of cells was analyzed using a theoretical model. The biomechanical results showed that the factor of viscosity of untreated HEK293T cells reduced from 0.65 to 0.40 for cells exposure to 40 μg/mL of AgNPs. Comet assay indicated that significant DNA damage occurred in the treated cells, measured as tail DNA% and tail moment. Furthermore, gene expression analysis showed that for the cells treated with 40 μg/mL of AgNPs, the antiapoptosis genes Bcl2-t and Bclw were, respectively, downregulated to 0.65- and 0.66-fold of control, and that the proapoptosis gene Bid was upregulated to 1.55-fold of control, which indicates that apoptosis occurred in cells exposed to AgNPs. Interestingly, excellent negative correlations were found between the factor of viscosity and tail DNA%, and tail moment, which suggest that the biomechanical property can be correlated with genotoxicity of nanoparticles on the cells. Based on the above results, we conclude that (1) AgNPs can lead to biomechanical changes in HEK293T cells, co...Continue Reading

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Citations

Aug 21, 2019·Archives of Toxicology·Gautam MahajanChandrasekhar Kothapalli
Aug 16, 2019·Pharmaceutical Research·Anastasiia B ShatanDaniel Horák
Apr 3, 2020·International Journal of Molecular Sciences·Zannatul Ferdous, Abderrahim Nemmar
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Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shafqat QamerSyafinaz Amin-Nordin

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Methods Mentioned

BETA
atomic force microscopy
AFM
force
optical microscopy
force measurement
Assay

Software Mentioned

Igor Pro
SPSS17
Origin
Matlab

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