Nanozyme chemiluminescence paper test for rapid and sensitive detection of SARS-CoV-2 antigen

Biosensors & Bioelectronics
Dan LiuXiyun Yan

Abstract

COVID-19 has evolved into a global pandemic. Early and rapid detection is crucial to control of the SARS-CoV-2 transmission. While representing the gold standard for early diagnosis, nucleic acid tests for SARS-CoV-2 are often complicated and time-consuming. Serological rapid antibody tests are characterized by high rates of false-negative diagnoses, especially during early infection. Here, we developed a novel nanozyme-based chemiluminescence paper assay for rapid and sensitive detection of SARS-CoV-2 spike antigen, which integrates nanozyme and enzymatic chemiluminescence immunoassay with the lateral flow strip. The core of our paper test is a robust Co-Fe@hemin-peroxidase nanozyme that catalyzes chemiluminescence comparable with natural peroxidase HRP and thus amplifies immune reaction signal. The detection limit for recombinant spike antigen of SARS-CoV-2 was 0.1 ng/mL, with a linear range of 0.2-100 ng/mL. Moreover, the sensitivity of test for pseudovirus could reach 360 TCID50/mL, which was comparable with ELISA method. The strip recognized SARS-CoV-2 antigen specifically, and there was no cross reaction with other coronaviruses or influenza A subtypes. This testing can be completed within 16 min, much shorter compared to...Continue Reading

References

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Citations

Jan 17, 2021·Journal of Clinical Medicine·Julien FavresseJonathan Douxfils
Jan 13, 2021·Biosensors & Bioelectronics·Quan-Quan ZhuangHao-Hua Deng
Mar 20, 2021·Biosensors & Bioelectronics·Jiuchuan GuoJinhong Guo
Mar 30, 2021·ACS Applied Materials & Interfaces·Mohammad Ali DerakhshanReza Faridi-Majidi
May 5, 2021·Journal of the American Chemical Society·Jinqi DengWeihong Tan
May 7, 2021·Biophysics and Physicobiology·Yuta KyoseiEtsuro Ito
May 29, 2021·ACS Applied Materials & Interfaces·Garrit KollerOwen Addison
Jun 3, 2021·Pathogens·Melanie FiedlerOlympia E Anastasiou
Jun 15, 2021·Chemical Reviews·Eka NovianaCharles S Henry
Sep 4, 2021·Analytical Biochemistry·Hoda IlkhaniSiamak Farhad
Sep 10, 2021·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yuan ZhouJian Wu
Nov 24, 2021·Mikrochimica Acta·Ceren KaramanMehmet Lütfi Yola

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Datasets Mentioned

BETA
MN908947

Methods Mentioned

BETA
PCR
nucleic acid amplification
biosensors
enzyme-linked immunosorbent assay
ELISA
Transmission
scanning electron microscopy
dynamic light scattering
Energy Dispersive Spectroscopy
X-ray

Software Mentioned

Pro Plus
Image

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