Naoxintong restores collateral blood flow in a murine model of hindlimb ischemia through PPARδ-dependent mechanism

Journal of Ethnopharmacology
Lu ChenHong Wang

Abstract

Naoxintong (NXT) is a compound preparation that is widely used in patients with cardiovascular and cerebrovascular diseases. The aim of this study is to investigate the protective mechanism of NXT on the mice model of peripheral vascular disease (PAD). In the study, hindlimb ischemia was induced by ligation of femoral artery on the right leg of mice. After surgery, the mice were administrated with saline solution, 10 mg/kg/d simvastatin and 700 mg/kg/d NXT for 4 weeks. The blood flow perfusion was measured by laser Doppler perfusion imaging system. Histological and immunofluorescent staining was used to determine muscle recovery, capillary density, tissue vascular endothelial growth factor (VEGF), phosphorylated-Akt (p-Akt) and phosphorylated-endothelial nitric oxide synthase (p-eNOS) expression. Terminal deoxynucleotidyl transferased UTP nick end labeling (TUNEL) was performed to detect the apoptosis of myocytes in hindlimb. The autophagy-associated gene expression and peroxisome proliferator-activated receptors (PPARs) expression were measured by Quantitative Real-Time Reverse Transcription Polymerase Chain Reaction (qRT-PCR). Western blotting was performed to detect the expressions of light-chain 3 (LC3), VEGF, p-Akt, p-eNOS...Continue Reading

Citations

May 7, 2020·Biotechnology and Applied Biochemistry·Jian ChenYong-Bing Cao
Aug 14, 2020·International Journal of Molecular Sciences·Nicole Wagner, Kay-Dietrich Wagner
Jan 15, 2021·Journal of Ethnopharmacology·Zhihao WangJing Tian

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