NAP1-RELATED PROTEIN1 and 2 negatively regulate H2A.Z abundance in chromatin in Arabidopsis.

Nature Communications
Yafei WangIsrael Ausin

Abstract

In eukaryotes, DNA wraps around histones to form nucleosomes, which are compacted into chromatin. DNA-templated processes, including transcription, require chromatin disassembly and reassembly mediated by histone chaperones. Additionally, distinct histone variants can replace core histones to regulate chromatin structure and function. Although replacement of H2A with the evolutionarily conserved H2A.Z via the SWR1 histone chaperone complex has been extensively studied, in plants little is known about how a reduction of H2A.Z levels can be achieved. Here, we show that NRP proteins cause a decrease of H2A.Z-containing nucleosomes in Arabidopsis under standard growing conditions. nrp1-1 nrp2-2 double mutants show an over-accumulation of H2A.Z genome-wide, especially at heterochromatic regions normally H2A.Z-depleted in wild-type plants. Our work suggests that NRP proteins regulate gene expression by counteracting SWR1, thereby preventing excessive accumulation of H2A.Z.

References

Feb 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yahli LorchRoger D Kornberg
Nov 9, 2007·The Plant Journal : for Cell and Molecular Biology·Rosana March-DíazJosé C Reyes
Feb 16, 2008·Structure·Jordanka Zlatanova, Amit Thakar
Sep 19, 2008·Genome Biology·Yong ZhangX Shirley Liu
Sep 26, 2008·Nature·Daniel ZilbermanSteven Henikoff
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Mar 4, 2010·Nature Reviews. Molecular Cell Biology·Paul B Talbert, Steven Henikoff
Oct 17, 2012·PLoS Genetics·Devin Coleman-Derr, Daniel Zilberman
Jan 28, 2014·Nature·Arnaud ObriAli Hamiche
Aug 26, 2014·Trends in Cell Biology·Paul B Talbert, Steven Henikoff
Apr 3, 2015·The Plant Journal : for Cell and Molecular Biology·Chi ZhangAiwu Dong
May 20, 2015·FEBS Letters·Amber R Cutter, Jeffrey J Hayes
Jun 3, 2015·Proceedings of the National Academy of Sciences of the United States of America·Ozge Gursoy-YuzugulluBrendan D Price
Jul 5, 2015·EMBO Reports·Hanan E Alatwi, Jessica A Downs
Dec 8, 2016·Nucleic Acids Research·Katiuska González-ArzolaIrene Díaz-Moreno
Dec 31, 2017·Proceedings of the National Academy of Sciences of the United States of America·Lihua ZhaoYuan Qin
Jan 25, 2018·Plant Science : an International Journal of Experimental Plant Biology·Balázs BarnaAttila Fehér
Jun 27, 2018·Nucleic Acids Research·Akihisa OsakabeFrédéric Berger

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Citations

Jan 13, 2021·Current Opinion in Plant Biology·Michael BorgFrédéric Berger
Jan 8, 2021·International Journal of Molecular Sciences·Fatemeh RasouliSergey Shabala
Jan 30, 2021·Frontiers in Plant Science·Sneha Lata BhadouriyaRajesh Mehrotra
May 4, 2021·FEBS Open Bio·Katiuska González-ArzolaMiguel A De la Rosa
Aug 30, 2021·The New Phytologist·Adam Hannan ParkerJurriaan Ton

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Methods Mentioned

BETA
acetylation
RNA-Seq
co-immunoprecipitation
co-IP
affinity purifications
immunoprecipitation
ChIP-Seq
ChIP-PCR
ChIP
bisulfite-Seq

Software Mentioned

deeptools
Studio
ChIPseeker
bsmap
Bowtie
ProLuCID
DTASelect2
Quant
MACS2

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