Naphthol-ASBI phosphate as a preferred substrate for tartrate-resistant acid phosphatase isoform 5b

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Anthony J JanckilaL T Yam

Abstract

Tartrate-resistant acid phosphatase (TRAP) isoform 5b is a potential serum marker for osteoclastic activity. Biochemical assays for serum TRAP activity with para-nitrophenylphosphate (pNPP) have low specificity for bone because of hydrolysis by unrelated nontype 5 TRAPs of blood cells and by related isoform 5a. Our purpose was to increase the specificity of TRAP assay for osteoclastic activity by using naphthol-ASBI phosphate (N-ASBI-P) as a substrate for serum type 5 TRAP activity and heparin as an inhibitor of isoform 5a. TRAP activity in individual and pooled sera of normal subjects and patients with endstage renal disease (ESRD) and rheumatologic diseases was quantitated using pNPP and N-ASBI-P as substrate at pH 5.5 and 6.1. For some experiments, heparin (23U/ml) was added as a specific inhibitor of isoform 5a activity. Isoforms 5a and 5b were separated from serum pools by cation exchange chromatography and identified by nondenaturing polyacrylamide gel electrophoresis (PAGE). N-ASBI-P was selectively hydrolyzed by TRAP isoform 5b. TRAP assays with pNPP and N-ASBI-P correlated only in ESRD sera, which contained primarily isoform 5b. The two assays did not correlate in normal or rheumatic sera with significant amounts of 5a...Continue Reading

References

Jul 1, 1978·American Journal of Clinical Pathology·A J JanckilaL T Yam
Aug 1, 1990·The Journal of Clinical Endocrinology and Metabolism·M E KraenzlinD J Baylink
Jan 24, 1973·Clinica Chimica Acta; International Journal of Clinical Chemistry·C P Modder
Jul 1, 1970·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C Y LiK W Lam
May 1, 1971·Analytical Chemistry·A VaughanD Hackney
Feb 23, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J M HalleenK Pettersson
Jul 14, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J M HalleenH K Väänänen

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Citations

Apr 4, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hyunil HaJin Yeul Ma
Apr 16, 2002·Molecular Pathology : MP·H BullP N Nelson
Oct 11, 2008·The Journal of Clinical Investigation·Antonios O AliprantisLaurie H Glimcher
Oct 31, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jing WuAntonios O Aliprantis
Jan 4, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Sari L AlataloJussi M Halleen
Mar 29, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Marie K LindbergClaes Ohlsson
Apr 30, 2013·Journal of Materials Science. Materials in Medicine·A BernhardtM Gelinsky
Nov 17, 2009·Calcified Tissue International·Anthony J Janckila, Lung T Yam
Apr 19, 2007·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·J LenoraP Gerdhem
Oct 25, 2008·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·J LenoraK K Ivaska
Dec 15, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·V GopalakrishnanN Srinivasan
Aug 8, 2007·International Urology and Nephrology·Barbara Santarosa Emo PetersLígia Araújo Martini
Sep 30, 2010·The Journal of Veterinary Medical Science·Danil KimShuichi Ohashi
Feb 19, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Shuichi MiyazakiTsugikazu Komoda
Mar 1, 2016·Bioorganic & Medicinal Chemistry Letters·Toshio KanedaHiroshi Morita
Oct 16, 2015·The Journal of Veterinary Medical Science·Kaoru HatateNorio Yamagishi
Sep 15, 2015·Journal of Medicinal Food·Jonggun KimKwang-Youn Whang
Mar 21, 2007·American Journal of Clinical Pathology·Anthony J JanckilaLung T Yam
Jan 5, 2010·The Journal of Veterinary Medical Science·Norio YamagishiKazuhisa Furuhama
Jun 13, 2014·Tissue Engineering. Part C, Methods·Anne BernhardtMichael Gelinsky
Mar 8, 2005·Blood Reviews·Evangelos TerposAmin Rahemtulla
Jun 7, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Marit-Naomi DugardM J Marshall
Feb 12, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Carina BlumUwe Gbureck
Aug 19, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Anthony J JanckilaLung T Yam
Dec 17, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Arja NenonenJussi M Halleen
Jan 10, 2013·American Journal of Physiology. Endocrinology and Metabolism·Ha-Young KimSubburaman Mohan
Jun 12, 2014·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Tomoyuki MukaiYasuyoshi Ueki
May 27, 2020·Biomedizinische Technik. Biomedical Engineering·Claus MosekeAndrea Ewald
Sep 18, 2020·International Journal of Molecular Sciences·Chia-Hung YenHui-Hua Hsiao
Sep 11, 2019·Materials Science & Engineering. C, Materials for Biological Applications·Boonlom ThavornyutikarnTerence W Turney
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yoshihiko IgarashiShigeru Matsuzaki
Feb 1, 2005·Archives of Biochemistry and Biophysics·Yunling WangGöran Andersson

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