Napsin A is negatively associated with EMT‑mediated EGFR‑TKI resistance in lung cancer cells

Molecular Medicine Reports
Linshui ZhouTingzhen Xu

Abstract

Epidermal growth factor receptor tyrosine kinase inhibitors (EGFR‑TKI) have been used as a standard therapy for patients with lung cancer with EGFR‑activating mutations. Epithelial‑mesenchymal transition (EMT) has been reported to be associated with the development of EGFR‑TKI resistance, which limits the clinical efficacy of EGFR‑TKI. Therefore, investigating the resistance‑associated mechanism is required in order to elucidate an effective therapeutic approach to enhance the sensitivity of lung cancer to EGFR‑TKI. In the present study, EGFR‑TKI erlotinib‑sensitive H358, H322 and H441 lung cancer cells, erlotinib‑moderately sensitive A549 cells, and erlotinib‑insensitive HCC827 cells with EGFR‑mutation (exon 19 deletion) were used to detect the mRNA and protein expression of the EMT‑associated proteins E‑cadherin and vimentin, and napsin A, by reverse transcription‑quantitative polymerase chain reaction analysis and western blotting. It was observed that the E‑cadherin expression level in erlotinib‑sensitive cells was increased compared with the moderately sensitive A549 cells and HCC827 cells; however, vimentin exhibited opposite expression, suggesting a correlation between EMT and erlotinib sensitivity in lung cancer cells. ...Continue Reading

References

Jan 1, 1996·Annual Review of Cell and Developmental Biology·E Ruoslahti
Dec 2, 1999·FEBS Letters·V Schauer-VukasinovicT Giller
May 12, 2000·Nature Cell Biology·D J SiegD D Schlaepfer
Oct 26, 2000·Japanese Journal of Cancer Research : Gann·T HiranoH Kato
Mar 30, 2001·The Journal of Biological Chemistry·B XieX Y Fu
Jun 7, 2002·IUBMB Life·Christof R HauckDavid D Schlaepfer
Jul 23, 2003·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Takashi HiranoHarubumi Kato
Aug 19, 2003·Nature Reviews. Molecular Cell Biology·Stefan GrünertHartmut Beug
Feb 10, 2004·The Journal of Biological Chemistry·Takayuki UenoTimothy E Weaver
Jun 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sanae NakajimaMasayuki Imamura
Nov 2, 2005·Pathology, Research and Practice·Akihiro SuzukiNoriyuki Sato
Aug 4, 2006·Cancer Research·Guillaume RobertSophie Tartare-Deckert
Jun 15, 2007·Pathology·Marcello GuarinoGianmario Ballabio
Jan 16, 2008·Laboratory Investigation; a Journal of Technical Methods and Pathology·Takayuki UenoStig Linder
Jun 10, 2008·Developmental Cell·Jing Yang, Robert A Weinberg
Dec 1, 2009·Cell·Jean Paul ThieryM Angela Nieto
Aug 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Zhan YaoRaffaella Sordella
Nov 29, 2011·Cancer Treatment Reviews·Jean-Charles SoriaPasi A Jänne
Nov 23, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Dana S Neel, Trever G Bivona

❮ Previous
Next ❯

Citations

Jul 15, 2021·Pathology Oncology Research : POR·Sören WeidemannSarah Minner

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.