Nasal epithelial cells of donor origin after allogeneic hematopoietic cell transplantation are generated at a faster rate in the first 3 months compared with later posttransplantation

Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation
Faisal M KhanJan Storek

Abstract

Detection of donor-type epithelial cells (ECs) after allogeneic hematopoietic cell transplantation (allo-HCT) using XY chromosome fluorescein in situ hybridization (FISH) has suggested that hematopoietic stem cells carry a degree of developmental plasticity. This is controversial, given artifacts of XY-based detection and the possibility of hematopoietic-nonhematopoietic cell fusion. Moreover, the kinetics of donor-type ECs (quantity at different time points after transplant) is unknown. Here, we document unequivocally the existence of donor-type ECs using a method obviating the artifacts of XY-FISH and study their kinetics. Nasal scrapings and blood specimens were collected from 60 allo-HCT survivors between 7 days and 22 years posttransplantation. DNA extracted from laser-captured nasal ECs (ie, CK(+)CD45(-) cells) and blood leukocytes was polymerase chain reaction-amplified for a panel of 16 short tandem repeat markers. The median percentage of donor-type ECs (among nasal ECs) was 0% on day 7 posttransplantation, 2.8% at 3 months posttransplantation, and 8.5% at 12-22 years posttransplantation. Cell fusion was ruled out by FISH analysis for two autosomes. We conclude that donor-type nasal ECs exist after HCT, and that their ...Continue Reading

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Citations

Apr 21, 2012·Current Opinion in Hematology·Mark BoschJan Storek
Mar 22, 2015·Cytotherapy·Christopher Nevala-PlagemannJakub Tolar
Mar 25, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Beau R Webber, Jakub Tolar
Dec 23, 2017·Journal of Cellular Physiology·Yuqing ZhuAhmed El-Hashash

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