Nasal mucus glutathione transferase activity and impact on olfactory perception and neonatal behavior

Scientific Reports
Aline Robert-HazotteJean-Marie Heydel

Abstract

In olfaction, to preserve the sensitivity of the response, the bioavailability of odor molecules is under the control of odorant-metabolizing enzymes (OMEs) expressed in the olfactory neuroepithelium. Although this enzymatic regulation has been shown to be involved in olfactory receptor activation and perceptual responses, it remains widely underestimated in vertebrates. In particular, the possible activity of OMEs in the nasal mucus, i.e. the aqueous layer that lined the nasal epithelium and forms the interface for airborne odorants to reach the olfactory sensory neurons, is poorly known. Here, we used the well-described model of the mammary pheromone (MP) and behavioral response in rabbit neonates to challenge the function of nasal mucus metabolism in an unprecedented way. First, we showed, in the olfactory epithelium, a rapid glutathione transferase activity toward the MP by ex vivo real-time mass spectrometry (PTR-MS) which supported an activity in the closest vicinity of both the odorants and olfactory receptors. Indeed and second, both the presence and activity of glutathione transferases were evidenced in the nasal mucus of neonates using proteomic and HPLC analysis respectively. Finally, we strikingly demonstrated that ...Continue Reading

References

Dec 31, 1965·Science·M X ZarrowC O Anderson
Aug 1, 1997·Chemical Senses·M GaribottiP Pelosi
Apr 4, 2000·Toxicological Sciences : an Official Journal of the Society of Toxicology·P S SamiecD P Jones
Dec 16, 2003·Brain Research·Gwendolyn K Whitby-LoganEric Walters
May 20, 2004·Chemical Senses·Gérard CoureaudBenoist Schaal
Oct 10, 2006·Current Biology : CB·Gérard CoureaudBenoist Schaal
Mar 27, 2007·Journal of Proteome Research·Hélène DébatJean-Claude Pernollet
Jun 13, 2008·The European Journal of Neuroscience·Nanaho FukudaKatsuhiko Mikoshiba
Jun 25, 2010·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Gérard CoureaudBenoist Schaal
Jul 20, 2010·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Nicolas ThiebaudAnne-Marie Le Bon
Dec 3, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ayumi Nagashima, Kazushige Touhara
Aug 1, 2007·Animal : an International Journal of Animal Bioscience·G CoureaudB Schaal
Aug 3, 2013·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Jean-Marie HeydelLoïc Briand
Jul 6, 2014·Proceedings. Biological Sciences·Gérard CoureaudGuillaume Ferreira
Oct 9, 2015·Chemical Senses·Philippe FaureJean-Marie Heydel
Jan 13, 2016·Analytical and Bioanalytical Chemistry·Rachel SchoumackerJean-Luc Le Quéré
May 10, 2017·Biological Reviews of the Cambridge Philosophical Society·Paolo PelosiFrancesca R Dani
Sep 2, 2017·Scientific Reports·Masashi AsakawaMasafumi Yohda
Nov 21, 2018·Journal of Agricultural and Food Chemistry·Eric Block
Nov 23, 2018·Scientific Reports·Keiichi YoshikawaPamela Dalton
Feb 23, 2019·Scientific Reports·Aline Robert-HazotteJean-Marie Heydel

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Citations

Jul 25, 2019·PloS One·Jean-Marie HeydelFabrice Neiers
Aug 22, 2020·Chemical Senses·Mathieu SchwartzFabrice Neiers
Dec 29, 2019·International Journal of Molecular Sciences·Alessandro MicarelliMarco Alessandrini
Jun 18, 2019·Drug Metabolism Reviews·Jean-Marie HeydelFabrice Neiers
Jun 30, 2019·Chemical Senses·Chiori IjichiKazushige Touhara
Dec 5, 2020·International Forum of Allergy & Rhinology·Zachary M SolerLauren E Ball
Oct 16, 2020·Chemistry : a European Journal·Tunga SalthammerWolf-Ulrich Palm
Oct 13, 2021·International Forum of Allergy & Rhinology·Chiori IjichiKenji Kondo
Oct 16, 2021·Comprehensive Reviews in Food Science and Food Safety·Mathieu SchwartzFrancis Canon

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Methods Mentioned

BETA
dissection
optical microscopy
dissections
acetylation

Software Mentioned

Elements Basic Research
DAQ
Chromeleon
Nis
Mascot
TOF

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