Nascent RNA synthesis in the context of chromatin architecture

Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology
Nicolas Sadoni, Daniele Zink

Abstract

Based on the idea that chromatin domains provide physical barriers for large molecules and multi-enzyme complexes, including the components of the transcription machinery, it has been proposed that transcription should be confined to the surfaces of chromatin domains. As a consequence nascent RNA should accumulate in the interchromatin space, which is thought to provide a special nuclear compartment involved in transcription, as well as in the processing and export of RNA (Cremer et al. 1993, Cremer & Cremer 2001). To further address the relationships between chromatin organization and RNA synthesis, we investigated the localization of BrUTP-labelled nascent RNA in HeLa cells stably expressing green fluorescent protein (GFP)-tagged histone H2B, which highlights the chromatin structure. Our results showed that nascent RNA does not preferentially localize within the interchromatin space. The findings do not support the idea that the interchromatin space provides a nuclear compartment playing an essential role in nascent RNA synthesis. However, the results are in agreement with the emerging view that even condensed chromatin domains display a highly dynamic organization and are not a physical barrier for transcription factors.

Citations

Sep 18, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Liudmila V SolovjevaNikolai V Tomilin
Oct 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Cindy Y Jao, Adrian Salic
Jan 19, 2006·The Journal of Cell Biology·Jill M BrownVeronica J Buckle
Sep 12, 2008·Critical Reviews in Oncology/hematology·Pavel TchélidzéDominique Ploton
May 1, 2007·Current Opinion in Cell Biology·Edith Heard, Wendy Bickmore
May 25, 2007·Nature·Peter Fraser, Wendy Bickmore
Feb 11, 2010·Izvestiia Akademii nauk. Seriia biologicheskaia·V M Manteĭfel', T I Karu
Feb 7, 2020·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Sajal Medha K AkkipeddiDawn M Carone
Apr 21, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eleni KtistakiDimitris Kioussis
May 23, 2008·Journal of Cell Science·Christopher H EskiwPeter R Cook

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