Nasopharyngeal culture with quantitative analysis of pathogenes in chronic otitis media with effusion. Effects on pathogen yield of different swabs and transport methods

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
C HemlinB Wretlind

Abstract

The influence of different swabs and transport media on nasopharyngeal culture pathogen recovery has been studied in patients with chronic otitis media with effusion. Transport times of less than two hours have been used. Protecting the cotton wire swab with a polyethylene shealth to prevent contamination by nasal flora did not have any significant influence either on the recovery of potential pathogens or on the contaminating nasal flora. Facilitating a quantitative analysis of the nasopharyngeal culture by transporting the specimen in empty tubes gave a pathogen recovery rate similar to that with transport in Stuart medium, whereas an attempt at transporting in sodium chloride or prereduced PY broth led to significantly lower yields of Branhamella catarrhalis (p less than 0.01) and in PY broth on Haemophilus influenzae as well (p less than 0.01).

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Citations

Nov 30, 1999·International Journal of Pediatric Otorhinolaryngology·I A Stewart
Feb 15, 2003·The Pediatric Infectious Disease Journal·Katherine L O'BrienUNKNOWN World Health Organization Pneumococcal Vaccine Trials Carraige Working Group
Feb 15, 2003·The Pediatric Infectious Disease Journal·Katherine L O'BrienUNKNOWN World Health Organization Pneumococcal Vaccine Trials Carriage Working Group
Oct 1, 1989·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·C HemlinB Wretlind

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