Native flatbed isoelectric focusing for determining antibody clonotype distribution.

Methods in Molecular Biology
B T Kurien, R H Scofield

Abstract

A sensitive method to analyze specific antibody clonotype changes in a patient with systemic lupus erythematosus, who developed autoantibodies to the Ro 60 autoantigen under observation, is described in this chapter. Patient sera collected over several years were separated by flatbed isoelectric focusing (IEF). Following electrofocusing, the gel is now ready to be analyzed by affinity immunoblotting utilizing Ro 60-coated nitrocellulose membrane to determine oligoclonality of the anti-Ro 60-containing sera.

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.