Native human autoantibodies targeting GIPC1 identify differential expression in malignant tumors of the breast and ovary.

BMC Cancer
Victoria YavelskyLeslie I Lobel

Abstract

We have been studying the native humoral immune response to cancer and have isolated a library of fully human autoantibodies to a variety of malignancies. We previously described the isolation and characterization of two fully human monoclonal antibodies, 27.F7 and 27.B1, from breast cancer patients that target the protein known as GIPC1, an accessory PDZ-domain binding protein involved in regulation of G-protein signaling. Human monoclonal antibodies, 27.F7 and 27.B1, to GIPC1 demonstrate specific binding to malignant breast cancer tissue with no reactivity with normal breast tissue. The current study employs cELISA, flow cytometry, Western blot analysis as well as immunocytochemistry, and immunohistochemistry. Data is analyzed statistically with the Fisher one-tail and two-tail tests for two independent samples. By screening several other cancer cell lines with 27.F7 and 27.B1 we found consistently strong staining of other human cancer cell lines including SKOV-3 (an ovarian cancer cell line). To further clarify the association of GIPC1 with breast and ovarian cancer we carefully studied 27.F7 and 27.B1 using immunocytochemical and immunohistochemical techniques. An immunohistochemical study of normal ovarian tissue, benign, ...Continue Reading

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Citations

Jun 8, 2013·Experimental & Molecular Medicine·Masaru Katoh
Feb 11, 2010·Journal of Oncology·Benjamin Piura, Ettie Piura
Nov 30, 2010·Journal of Oncology·Ettie Piura, Benjamin Piura
Jan 7, 2011·PloS One·Thomas W ChittendenJohn Quackenbush
Nov 7, 2012·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·A SingerM Krause
Aug 3, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Guilong ZhangNing Gu
Jan 14, 2016·Journal of the National Cancer Institute·Jules A WestbrookJanet E Brown
Feb 11, 2015·Journal of Cell Science·Nicola De FranceschiJohanna Ivaska

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Methods Mentioned

BETA
gel filtration
ELISA
biopsies
flow cytometry
fluorescence microscopy
light microscopy
confocal microscopy

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