PMID: 7871385Mar 1, 1995

Native human serum amyloid P component is a single pentamer

Scandinavian Journal of Immunology
I J SørensenS E Svehag

Abstract

Serum amyloid P component (SAP) and C-reactive protein (CRP) are members of the pentraxin protein family. SAP is the precursor protein to amyloid P component present in all forms of amyloidosis. The prevailing notion is that SAP in circulation has the form of a double pentameric molecule (decamer) whereas CRP is a single pentameric molecule. We have investigated by gel permeation chromatography the M(r) of SAP in freshly collected human serum and of SAP purified by carbohydrate affinity chromatography and anion exchange chromatography. SAP was monitored by quantitative immunoelectrophoresis and ELISA, and SAP peak fractions were analysed by use of SDS-PAGE, Western blotting, and electron microscopy. The results indicate that native SAP circulates as a single pentamer, a part of which forms complexes with C4b-binding protein. The properties of SAP changed during purification as indicated by rocket immunoelectrophoresis and electron microscopy. Thus, electron micrographs of purified SAP showed a predominance of decamers. However, the decamer form of SAP reversed to single pentamers when purified SAP was incorporated into SAP-depleted serum.

References

Aug 1, 1996·Cytokine & Growth Factor Reviews·A R GoodmanJ Vilcek
Aug 1, 1997·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·M PalmS E Svehag
Mar 17, 2015·PloS One·Darrell PillingRichard H Gomer
Nov 20, 2014·Physical Chemistry Chemical Physics : PCCP·Mimi GaoSimon Ebbinghaus
Aug 1, 1996·European Journal of Biochemistry·N H H HeegaardF A Robey
Jan 20, 2006·European Journal of Immunology·Dietger StibenzChristoph Bührer

Citations

Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A P OsmandE Shelton
Jan 1, 1988·International Archives of Allergy and Applied Immunology·B M KubakL A Potempa
Mar 1, 1988·The Journal of Experimental Medicine·P N HawkinsM B Pepys
Oct 1, 1981·The Journal of Experimental Medicine·F C de BeerM B Pepys
Jan 1, 1982·Annals of the New York Academy of Sciences·R H PainterS B Stern
Jan 1, 1995·International Archives of Allergy and Immunology·I J SørensenS E Svehag
Jan 27, 1994·Nature·J EmsleyS P Wood

Related Concepts

Immunoelectrophoresis
Protein Family
C-reactive Protein Measurement
Amyloidosis
Western Blotting
SDS-PAGE
Complex (molecular entity)
Chromatography
Properdin Deficiency, X-LINKED
Biopolymers

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