Native-like SARS-CoV-2 spike glycoprotein expressed by ChAdOx1 nCoV-19/AZD1222 vaccine.

BioRxiv : the Preprint Server for Biology
Yasunori WatanabeMax Crispin

Abstract

Vaccine development against the SARS-CoV-2 virus focuses on the principal target of the neutralizing immune response, the spike (S) glycoprotein. Adenovirus-vectored vaccines offer an effective platform for the delivery of viral antigen, but it is important for the generation of neutralizing antibodies that they produce appropriately processed and assembled viral antigen that mimics that observed on the SARS-CoV-2 virus. Here, we describe the structure, conformation and glycosylation of the S protein derived from the adenovirus-vectored ChAdOx1 nCoV-19/AZD1222 vaccine. We demonstrate native-like post-translational processing and assembly, and reveal the expression of S proteins on the surface of cells adopting the trimeric prefusion conformation. The data presented here confirms the use of ChAdOx1 adenovirus vectors as a leading platform technology for SARS-CoV-2 vaccines.

Citations

Mar 17, 2021·Wiener klinische Wochenschrift·Franz X Heinz, Karin Stiasny
May 10, 2021·European Journal of Internal Medicine·Fabio AngeliPaolo Verdecchia
May 27, 2021·Scientific Reports·Margherita PassarielloClaudia De Lorenzo

Methods Mentioned

BETA
glycosylation
flow cytometry
biopsy
transfect
size-exclusion chromatography
affinity purification
gel filtration
PCR
electron microscopy

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