Native low density lipoprotein promotes lipid raft formation in macrophages

Molecular Medicine Reports
Jian SongJunzhu Wu

Abstract

Oxidized low‑density lipoprotein (LDL) has an important role in atherogenesis; however, the mechanisms underlying cell‑mediated LDL oxidation remain to be elucidated. The present study investigated whether native‑LDL induced lipid raft formation, in order to gain further insight into LDL oxidation. Confocal microscopic analysis revealed that lipid rafts were aggregated or clustered in the membrane, which were colocalized with myeloperoxidase (MPO) upon native LDL stimulation; however, in the presence of methyl‑β‑cyclodextrin (MβCD), LDL‑stimulated aggregation, translocation, and colocalization of lipid rafts components was abolished.. In addition, lipid raft disruptors MβCD and filipin decreased malondialdehyde expression levels. Density gradient centrifugation coupled to label‑free quantitative proteomic analysis identified 1,449 individual proteins, of which 203 were significantly upregulated following native‑LDL stimulation. Functional classification of the proteins identified in the lipid rafts revealed that the expression levels of translocation proteins were upregulated. In conclusion, the results of the present study indicated that native‑LDL induced lipid raft clustering in macrophages, and the expression levels of seve...Continue Reading

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Citations

Oct 5, 2018·Circulation Journal : Official Journal of the Japanese Circulation Society·Hiroki KatsutaHiromi Goto
Jun 9, 2021·Molecular Brain·Hajar Miranzadeh MahabadiChangiz Taghibiglou

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Methods Mentioned

BETA
MDA
electrophoresis
X-ray
confocal microscopy
density gradient centrifugation

Software Mentioned

Image J
GraphPad
GraphPad Prism
Sage Sorcerer SEQUEST

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