Native mass spectrometry identifies an alternative DNA-binding pathway for BirA from Staphylococcus aureus

Scientific Reports
Jiulia SatiaputraSteven W Polyak

Abstract

An adequate supply of biotin is vital for the survival and pathogenesis of Staphylococcus aureus. The key protein responsible for maintaining biotin homeostasis in bacteria is the biotin retention protein A (BirA, also known as biotin protein ligase). BirA is a bi-functional protein that serves both as a ligase to catalyse the biotinylation of important metabolic enzymes, as well as a transcriptional repressor that regulates biotin biosynthesis, biotin transport and fatty acid elongation. The mechanism of BirA regulated transcription has been extensively characterized in Escherichia coli, but less so in other bacteria. Biotin-induced homodimerization of E. coli BirA (EcBirA) is a necessary prerequisite for stable DNA binding and transcriptional repression. Here, we employ a combination of native mass spectrometry, in vivo gene expression assays, site-directed mutagenesis and electrophoretic mobility shift assays to elucidate the DNA binding pathway for S. aureus BirA (SaBirA). We identify a mechanism that differs from that of EcBirA, wherein SaBirA is competent to bind DNA as a monomer both in the presence and absence of biotin and/or MgATP, allowing homodimerization on the DNA. Bioinformatic analysis demonstrated the SaBirA se...Continue Reading

References

Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·K P WilsonB W Matthews
Sep 7, 2000·Protein Science : a Publication of the Protein Society·K Kwon, D Beckett
Nov 21, 2001·Protein Science : a Publication of the Protein Society·A Chapman-SmithJ C Wallace
Oct 9, 2002·Genome Research·Dmitry A RodionovMikhail S Gelfand
Jan 16, 2003·Journal of Molecular Biology·Emily D Streaker, Dorothy Beckett
Apr 2, 2004·The Journal of Biological Chemistry·Charlotte L Hanson, Carol V Robinson
Jun 10, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Nicole R PendiniMatthew C J Wilce
Oct 23, 2008·Nucleic Acids Research·Eric W SayersJian Ye
Apr 14, 2009·Journal of Molecular Biology·Huaying ZhaoDorothy Beckett
Oct 13, 2011·Molecular Systems Biology·Fabian SieversDesmond G Higgins
Jan 3, 2012·Journal of Bacteriology·Vandana Chakravartty, John E Cronan
Mar 1, 2012·Bioinformatics·Konstantin OkonechnikovUNKNOWN UGENE team
Mar 23, 2012·The Journal of Biological Chemistry·Tatiana P Soares da CostaAndrew D Abell
Mar 27, 2012·Journal of Molecular Biology·Poorni R Adikaram, Dorothy Beckett
Apr 6, 2013·Protein Science : a Publication of the Protein Society·Nicole R PendiniMatthew C J Wilce
Jul 31, 2013·Journal of Molecular Biology·Poorni R Adikaram, Dorothy Beckett
Dec 10, 2013·Molecular Microbiology·Youjun FengJohn E Cronan
Dec 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·David G PriestKeith E Shearwin
Sep 7, 2014·Bioorganic & Medicinal Chemistry Letters·William TieuAndrew D Abell
Mar 10, 2015·Journal of Molecular Biology·Christopher EgintonDorothy Beckett
May 4, 2017·Protein Science : a Publication of the Protein Society·Jingheng Wang, Dorothy Beckett
Jul 19, 2017·Biochemistry·Jingheng WangSilvina Matysiak
Feb 5, 2016·Synthetic and Systems Biotechnology·J SatiaputraS W Polyak
Nov 14, 2017·ACS Infectious Diseases·Ashleigh S PaparellaAndrew D Abell
Mar 7, 2018·Applied Microbiology and Biotechnology·Jiulia SatiaputraSteven W Polyak

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Methods Mentioned

BETA
electrophoretic mobility shift
X-ray

Software Mentioned

UGENE
UCSF Chimera
MassLynx

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