Abstract
Direct protein delivery into intact plants remains a challenge for the agricultural and plant science fields. Cell-penetrating peptide (CPP)-mediated protein delivery requires the binding of CPPs to a protein to carry the protein into the cell through the cell wall and lipid bilayer. Thus, we prepared ionic complexes of a CPP-containing carrier peptide and a cargo protein, namely, Citrine yellow fluorescent protein, and subsequently studied their physicochemical properties. Two types of carrier peptides, BP100(KH)9 and BP100CH7, were investigated for delivery efficiency into rice callus. Both BP100(KH)9 and BP100CH7 successfully introduced Citrine protein into rice callus cells under pressure and vacuum treatment. Moreover, delivery efficiency varied at different growth stages of rice callus; 5-day rice callus was a more efficient recipient for Citrine than 21-day callus.
References
Aug 1, 1994·The Plant Journal : for Cell and Molecular Biology·Y HieiT Kumashiro
Apr 3, 1998·Human Gene Therapy·S L HartC Kinnon
Jul 21, 2004·Journal of Structural and Functional Genomics·Takanori KigawaShigeyuki Yokoyama
Nov 2, 2005·Nature Reviews. Molecular Cell Biology·Daniel J Cosgrove
Jun 1, 1977·Plant Physiology·M C Meinert, D P Delmer
Sep 12, 2006·The Plant Journal : for Cell and Molecular Biology·Seiichi TokiHiroshi Tanaka
Dec 15, 2010·Chembiochem : a European Journal of Chemical Biology·Kai EggenbergerPeter Nick
Sep 6, 2012·BMC Plant Biology·Anna NadalMaria Pla
Oct 10, 2012·Biomacromolecules·Keiji NumataYoshiharu Doi
Dec 12, 2012·Biomacromolecules·Manoj LakshmananKeiji Numata
May 15, 2013·FEBS Letters·Chérine Bechara, Sandrine Sagan
Aug 10, 2013·Nature Biotechnology·Wei LiQi Zhou
Sep 4, 2013·Nucleic Acids Research·Wenzhi JiangDonald P Weeks
Jan 1, 2014·PloS One·Esther BadosaEmilio Montesinos
Jun 7, 2014·Plant Biotechnology Journal·Keiji NumataYutaka Kodama
Nov 14, 2014·Plant & Cell Physiology·Masaki EndoSeiichi Toki
Apr 23, 2016·PloS One·Kiaw Kiaw NgKeiji Numata
Apr 22, 2016·Plant Cell Reports·Chidananda Nagamangala Kanchiswamy
Nov 17, 2016·Nature Communications·Sergei SvitashevA Mark Cigan
Mar 16, 2017·BMC Plant Biology·Laura MontesinosEmilio Montesinos
Mar 30, 2017·Scientific Reports·Vladimir NekrasovSophien Kamoun
Mar 3, 2018·Biomacromolecules·Jo-Ann Chuah, Keiji Numata
Jul 22, 2018·Scientific Reports·Keiji NumataMisato Ohtani
Jan 1, 2016·Plant Biotechnology·Keiji NumataYutaka Kodama
Citations
Aug 23, 2020·Plants·Yuki YanagawaIchiro Mitsuhara
Nov 20, 2020·International Journal of Molecular Sciences·Mieko Higuchi-TakeuchiKeiji Numata
Dec 15, 2020·Biomacromolecules·Seiya FujitaKeiji Numata
Mar 18, 2021·Transgenic Research·Rishikesh GhogareAmit Dhingra
Aug 14, 2020·Biomacromolecules·Riku KawasakiKeiji Numata
Apr 27, 2021·ACS Biomaterials Science & Engineering·Kenta WatanabeKeiji Numata
Apr 27, 2021·Plant Communications·Yingxiao ZhangYiping Qi
May 14, 2021·Current Opinion in Plant Biology·Jeffrey W WangMarkita P Landry