Native protein delivery into rice callus using ionic complexes of protein and cell-penetrating peptides

PloS One
Boyang GuoKeiji Numata

Abstract

Direct protein delivery into intact plants remains a challenge for the agricultural and plant science fields. Cell-penetrating peptide (CPP)-mediated protein delivery requires the binding of CPPs to a protein to carry the protein into the cell through the cell wall and lipid bilayer. Thus, we prepared ionic complexes of a CPP-containing carrier peptide and a cargo protein, namely, Citrine yellow fluorescent protein, and subsequently studied their physicochemical properties. Two types of carrier peptides, BP100(KH)9 and BP100CH7, were investigated for delivery efficiency into rice callus. Both BP100(KH)9 and BP100CH7 successfully introduced Citrine protein into rice callus cells under pressure and vacuum treatment. Moreover, delivery efficiency varied at different growth stages of rice callus; 5-day rice callus was a more efficient recipient for Citrine than 21-day callus.

References

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Citations

Nov 20, 2020·International Journal of Molecular Sciences·Mieko Higuchi-TakeuchiKeiji Numata
Mar 18, 2021·Transgenic Research·Rishikesh GhogareAmit Dhingra
Apr 27, 2021·ACS Biomaterials Science & Engineering·Kenta WatanabeKeiji Numata
Apr 27, 2021·Plant Communications·Yingxiao ZhangYiping Qi
May 14, 2021·Current Opinion in Plant Biology·Jeffrey W WangMarkita P Landry

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Methods Mentioned

BETA
transgenic
electrophoresis
genetic modification
genetic modifications

Software Mentioned

Imaris
ImageJ

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