Natriuretic peptide-augmented induction of nitric oxide synthase through cyclic guanosine 3',5'-monophosphate elevation in vascular smooth muscle cells

Endocrinology
T MarumoT Saruta

Abstract

To elucidate the role of natriuretic peptides in vascular remodeling, the effects of atrial natriuretic peptide, brain natriuretic peptide, and C-type natriuretic peptide (CNP) on the induction of inducible nitric oxide (NO) synthase (iNOS) in rat aortic smooth muscle cells were examined. Although none of the peptides when applied alone induced the production of nitrite, a stable end product of NO, each peptide dramatically enhanced nitrite production induced by a cytokine combination of interleukin-1 alpha and tumor necrosis factor-alpha. Each natriuretic peptide stimulated intracellular cGMP accumulation in a dose-dependent manner. Time-dependent nitrite production by the cytokines was increased by CNP cotreatment and inhibited by NG-methyl-L-arginine, indicating involvement of the L-arginine-NO pathway. Northern blot analysis showed that the augmented nitrite production was accompanied by an increase in iNOS messenger RNA. A cGMP analog, 8-bromo-cGMP, completely mimicked all of the effects of CNP described above. A cGMP-dependent protein kinase inhibitor, KT5823, paradoxically increased nitrite production and iNOS messenger RNA levels induced by the combination of 8-bromo-cGMP and both cytokines or by the two cytokines only....Continue Reading

Citations

Jun 7, 2005·Neuropeptides·Veena PuriNancy E J Berman
Nov 22, 2001·Current Hypertension Reports·L V d'Uscio, T F Lüscher
Dec 10, 1999·European Journal of Obstetrics, Gynecology, and Reproductive Biology·J EgbertsP Soederhuizen
May 30, 1998·British Journal of Pharmacology·H KleinertU Förstermann
Feb 24, 2009·The Annals of Thoracic Surgery·Hao G NguyenOz M Shapira
Nov 11, 2008·Journal of Cardiac Failure·Yutaka IshibashiToshio Shimada
Feb 25, 2009·Atherosclerosis·Emanuele BarbatoMassimo Volpe
Jul 24, 2010·British Journal of Pharmacology·Catherine M PanayiotouAdrian J Hobbs
Sep 19, 2000·Free Radical Research·S ParthasarathyO Meilhac
Dec 5, 2003·Biochimica Et Biophysica Acta·Sergio de FrutosM Rodriguez-Puyol
Jul 8, 2008·Autonomic Neuroscience : Basic & Clinical·Geisa C S V TeziniHugo C D Souza
Jan 11, 2005·Rheumatic Diseases Clinics of North America·Sergio GeneriniMarco Matucci-Cerinic
Jun 2, 2005·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Helen M Bramlett
Dec 30, 2008·The Journal of Surgical Research·Brenda S ChoGilbert R Upchurch
Jan 6, 2009·Toxicology and Applied Pharmacology·Richard P WyethJon E Sprague
Dec 17, 2005·International Journal of Cardiology·Zenon S KyriakidesDimitri Th Kremastinos
Apr 18, 2009·Molecular Aspects of Medicine·Tobias JungTilman Grune
Jul 19, 2001·Japanese Journal of Pharmacology·D A Pelligrino, E Galea
May 29, 2007·Biochemical and Biophysical Research Communications·María Angeles CostaCristina Arranz
Mar 29, 2002·Annals of Neurology·Rajani ChoudhuriNancy E J Berman
Aug 28, 2003·Clinical and Experimental Hypertension : CHE·Mohammad A NewazAdebayo Oyekan
Feb 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·M B HussainA J Hobbs

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