Natriuretic peptide gene expression after beta-adrenergic stimulation in adult mouse cardiac myocytes

DNA and Cell Biology
Aziz N AnderDavid M Roth

Abstract

The role of A- and B-type natriuretic peptides (ANP and BNP) in cardiac pathophysiology are of increasing interest. Isolated neonatal mouse cardiac myocytes express increased levels of ANP mRNA in the absence of growth factors in culture. Expression of ANP and BNP mRNA has not been studied in isolated adult mouse cardiac myocytes (AMCM). We examined expression of ANP and BNP mRNA in isolated AMCM with and without stimulation with beta-adrenergic receptor agonists and antagonists. AMCM were isolated and maintained in culture for 24-48 h with and without stimulation with the beta-adrenergic receptor agonist isoproterenol (Iso), the beta1-antagonist CGP20712A (CGP), or the beta2-antagonist ICI-118,551 (ICI). Northern blot analysis was performed using probes for mouse ANP and BNP mRNA. TUNEL assay was performed after beta-adrenergic receptor stimulation of AMCM. BNP mRNA expression was increased fivefold (P < 0.001) after 48 h in culture without adrenergic stimulation. BNP mRNA expression was reduced (P < 0.0001) after stimulation with Iso while ANP expression remained similar to unstimulated cells. CGP prevented the Iso reduction in BNP mRNA. Iso stimulation at doses that reduced BNP mRNA expression increased TUNEL positive nuclei...Continue Reading

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Citations

Feb 3, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hemal H PatelDavid M Roth
Dec 24, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Aldo ClericoMichele Emdin
Feb 8, 2008·International Journal of Cardiology·Vasiliki BistolaManousos M Konstadoulakis

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