Natriuretic peptide receptor 3 (NPR3) is regulated by microRNA-100

Journal of Molecular and Cellular Cardiology
Lee Lee WongYei-Tsung Chen

Abstract

Natriuretic peptide receptor 3 (NPR3) is the clearance receptor for the cardiac natriuretic peptides (NPs). By modulating the level of NPs, NPR3 plays an important role in cardiovascular homeostasis. Although the physiological functions of NPR3 have been explored, little is known about its regulation in health or disease. MicroRNAs play an essential role in the post-transcriptional expression of many genes. Our aim was to investigate potential microRNA-based regulation of NPR3 in multiple models. Hypoxic challenge elevated levels of NPPB and ADM mRNA, as well as NT-proBNP and MR-proADM in human left ventricle derived cardiac cells (HCMa), and in the corresponding conditioned medium, as revealed by qRT-PCR and ELISA. NPR3 was decreased while NPR1 was increased by hypoxia at mRNA and protein levels in HCMa. Down-regulation of NPR3 mRNA was also observed in infarct and peri-infarct cardiac tissue from rats undergoing myocardial infarction. From microRNA microarray analyses and microRNA target predictive databases, miR-100 was selected as a candidate regulator of NPR3 expression. Further analyses confirmed up-regulation of miR-100 in hypoxic cells and associated conditioned media. Antagomir-based silencing of miR-100 enhanced NPR3 ...Continue Reading

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Citations

Mar 15, 2016·Molecular and Cellular Endocrinology·Sharon Li Ting PekSubramaniam Tavintharan
Apr 9, 2016·International Journal of Molecular Sciences·Lee Lee WongYei-Tsung Chen
May 24, 2016·International Journal of Molecular Sciences·Juan WangYei-Tsung Chen
May 24, 2016·International Journal of Molecular Sciences·Yei-Tsung ChenLieng-Hsi Ling
Jan 19, 2018·Journal of Biological Engineering·Shihui FuLeiming Luo
Apr 1, 2016·Physiological Reviews·Michaela Kuhn
Jun 20, 2020·Cellular and Molecular Life Sciences : CMLS·Speranza RubattuMaurizio Forte
Sep 16, 2020·Immunological Investigations·Zahra SaadatianMir Davood Omrani
Oct 5, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christian SmolkaFranziska Pankratz

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