PMID: 8596701Feb 1, 1996Paper

Natriuretic peptides increase a K+ conductance in rat mesangial cells

Pflügers Archiv : European journal of physiology
R CermakE Schlatter

Abstract

Mesangial cells (MC) are a main target of natriuretic peptides in the kidney and are thought to play a role in regulating glomerular filtration rate. We examined the influence of cGMP-generating (i.e. guanosine 3',5'-cyclicmonophosphate) peptides on membrane voltages (Vm) of rat MC by using the fast whole-cell patch-clamp technique. The cGMP-generating peptides were tested at maximal concentrations ranging from 140 to 300 nmol/l. Whereas human CNP (C natriuretic peptide), rat guanylin and human uroguanylin had no significant effect on Vm these cells, human BNP (brain natriuretic peptide), rat CDD/ANP-99-126 (cardiodilatin/atrial natriuretic peptide) and rat CDD/ANP-95-126 (urodalatin) hyperpolarized Vm significantly by 1.6 +/- 0.4 mV (BNP, n=8), 3.7 +/- 0.3 mV (CDD/ANP-99-126, n=25) and 2.8 +/- 0.4 mV (urodilatin, n=9), respectively. The half-maximally effective concentration (EC50) for the latter two was around 400 pmol/l each. This hyperpolarization could be mimicked with 0.5 mmol/l 8-bromo-guanosine 3',5'-cyclic monophosphate (8-Br-cGMP) and was blocked by 5 mmol/l Ba2+. The K+ channel blocker 293 B (100 micromol/l) depolarized basal Vm by 4.3 +/- 0.4 mV (n=8), but failed to inhibit the hyperpolarization induced by CDD/ANP-9...Continue Reading

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Citations

Aug 6, 2011·Canadian Journal of Physiology and Pharmacology·Kailash N Pandey
Jul 20, 1999·Kidney International·J GloyH Pavenstädt
Aug 24, 1999·The Journal of Physiology·J R HirschE Schlatter
May 16, 2002·Microscopy Research and Technique·Hermann Pavenstädt, Martin Bek
Jul 1, 2008·Journal of the American Society of Hypertension : JASH·Kailash N Pandey
May 25, 2005·Peptides·Kailash N Pandey
Jan 26, 1999·Medicinal Research Reviews·J I Kourie, M J Rive
Jul 17, 1997·Nature·T V McDonaldG I Fishman
Sep 18, 2001·Canadian Journal of Physiology and Pharmacology·J I KourieC L Henry
Jul 23, 1998·Physiological Reviews·J D Stockand, S C Sansom
Jun 27, 1997·Biochemical and Biophysical Research Communications·T BillingtonC A Gehring

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