Natural and Artificial Strategies To Control the Conjugative Transmission of Plasmids

Microbiology Spectrum
María Getino, Fernando de la Cruz

Abstract

Conjugative plasmids are the main carriers of transmissible antibiotic resistance (AbR) genes. For that reason, strategies to control plasmid transmission have been proposed as potential solutions to prevent AbR dissemination. Natural mechanisms that bacteria employ as defense barriers against invading genomes, such as restriction-modification or CRISPR-Cas systems, could be exploited to control conjugation. Besides, conjugative plasmids themselves display mechanisms to minimize their associated burden or to compete with related or unrelated plasmids. Thus, FinOP systems, composed of FinO repressor protein and FinP antisense RNA, aid plasmids to regulate their own transfer; exclusion systems avoid conjugative transfer of related plasmids to the same recipient bacteria; and fertility inhibition systems block transmission of unrelated plasmids from the same donor cell. Artificial strategies have also been designed to control bacterial conjugation. For instance, intrabodies against R388 relaxase expressed in recipient cells inhibit plasmid R388 conjugative transfer; pIII protein of bacteriophage M13 inhibits plasmid F transmission by obstructing conjugative pili; and unsaturated fatty acids prevent transfer of clinically relevant ...Continue Reading

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Citations

Aug 8, 2018·FEMS Microbiology Reviews·Michelle M C BucknerLaura J V Piddock
Oct 22, 2018·Drug Development Research·Fabrice E GrafAnne Farewell
Sep 7, 2019·Genome Génome / Conseil National De Recherches Canada·Galen E CardRichard A Robison
Jan 26, 2020·FEMS Microbiology Reviews·Dan I AnderssonTone Tønjum
Dec 5, 2017·Frontiers in Microbiology·María GetinoFernando de la Cruz
Nov 30, 2019·Frontiers in Microbiology·M Pilar Garcillán-BarciaFernando de la Cruz
Sep 26, 2017·Microbiology Spectrum·Alvaro San Millan, R Craig MacLean
Jun 22, 2021·Frontiers in Microbiology·Kevin NeilSébastien Rodrigue
Aug 25, 2021·MBio·Carolina Palencia-GándaraFernando de la Cruz
Oct 5, 2021·Nucleic Acids Research·Rafael Pinilla-RedondoSøren J Sørensen
Nov 30, 2021·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Timothy M Ghaly, Michael R Gillings
Nov 30, 2021·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Claudia IglerSonja Lehtinen

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