PMID: 7541059Jun 1, 1995Paper

Natural and recombinant enzymatically active or inactive bee venom phospholipase A2 has the same potency to release histamine from basophils in patients with Hymenoptera allergy

The Journal of Allergy and Clinical Immunology
E FörsterM Suter

Abstract

A complementary DNA encoding the major bee venom allergen phospholipase A2 (PLA) has been characterized recently. Recombinant PLA was produced in Escherichia coli and purified to apparent homogeneity. Natural PLA was compared with recombinant PLA in its ability to release histamine from blood basophils. A synthetic gene encoding the mature form of PLA was expressed in E. coli, and the polypeptide was purified to homogeneity by affinity chromatography and refolded, yielding fully enzymatically active PLA. In addition, we have produced a genetically engineered enzymatically inactive variant by substitution of a single amino acid residue in the catalytic center. A standard histamine release assay was used to compare the potency of natural PLA with correctly folded enzymatically active and inactive recombinant PLA to release histamine from blood basophils of nine patients with bee venom allergy. Recombinant enzymatically active PLA and purified natural protein were equally effective in releasing histamine from sensitized basophils. By comparing the histamine-releasing capacity of enzymatically active and inactive recombinant allergen, we further demonstrate that catalytic activity is not a requirement for allergenicity in the effec...Continue Reading

References

Feb 1, 1976·Archives of Biochemistry and Biophysics·T P KingL M Lichtenstein
Jul 28, 1972·Science·E Habermann
Sep 1, 1995·The Journal of Allergy and Clinical Immunology·U R MüllerM Suter
Feb 1, 1993·The Journal of Allergy and Clinical Immunology·D W MacGlashan

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Citations

Jul 9, 2002·Allergy·U R Müller
Jul 25, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S M McHugh
Feb 7, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·B CalabozoF Polo
Dec 18, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P JohansenT M Kündig
Jul 4, 2015·Current Allergy and Asthma Reports·Markus Ollert, Simon Blank
May 18, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M OkanoK Nishizaki
Aug 28, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·D WymannK Blaser
Oct 2, 2004·Protein Science : a Publication of the Protein Society·Cécile BuhotBernard Maillère
Jul 25, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R KämmererF Spertini
Jun 27, 2019·Clinical Reviews in Allergy & Immunology·Abida ZahirovićMojca Lunder
Jul 17, 2003·Current Opinion in Allergy and Clinical Immunology·Ulrich R Müller

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