Natural and redesigned wasp venom peptides with selective antitumoral activity

Beilstein Journal of Organic Chemistry
Marcelo Der Torossian TorresVani X Oliveira

Abstract

About 1 in 8 U.S. women (≈12%) will develop invasive breast cancer over the course of their lifetime. Surgery, chemotherapy, radiotherapy, and hormone manipulation constitute the major treatment options for breast cancer. Here, we show that both a natural antimicrobial peptide (AMP) derived from wasp venom (decoralin, Dec-NH2), and its synthetic variants generated via peptide design, display potent activity against cancer cells. We tested the derivatives at increasing doses and observed anticancer activity at concentrations as low as 12.5 μmol L-1 for the selective targeting of MCF-7 breast cancer cells. Flow cytometry assays further revealed that treatment with wild-type (WT) peptide Dec-NH2 led to necrosis of MCF-7 cells. Additional atomic force microscopy (AFM) measurements indicated that the roughness of cancer cell membranes increased significantly when treated with lead peptides compared to controls. Biophysical features such as helicity, hydrophobicity, and net positive charge were identified to play an important role in the anticancer activity of the peptides. Indeed, abrupt changes in peptide hydrophobicity and conformational propensity led to peptide inactivation, whereas increasing the net positive charge of peptides...Continue Reading

References

Jan 1, 1984·Annual Review of Biochemistry·D Eisenberg
Jul 2, 1998·Biophysical Journal·C N Pace, J M Scholtz
Dec 15, 2007·Biochimica Et Biophysica Acta·David W Hoskin, Ayyalusamy Ramamoorthy
Apr 22, 2008·Current Opinion in Chemical Biology·Josef VagnerVictor J Hruby
Oct 20, 2009·European Journal of Pharmacology·Frank Schweizer
Oct 1, 2011·Biomacromolecules·Jing HuJian R Lu
Dec 17, 2011·International Journal of Molecular Sciences·Sammy Al-BennaLars Steinstraesser
Jul 24, 2012·Marine Drugs·Guadalupe-Miroslava Suarez-JimenezJosafat-Marina Ezquerra-Brauer
Jul 31, 2013·Journal of Peptide Science : an Official Publication of the European Peptide Society·Mayra ChamlianVani X Oliveira
Feb 11, 2014·Peptides·Brij Pal SinghSubrota Hati
May 13, 2014·Journal of Peptide Science : an Official Publication of the European Peptide Society·Adriana Farias SilvaVani Xavier Oliveira
Nov 26, 2014·Journal of Peptide Science : an Official Publication of the European Peptide Society·Marcelo Der Torossian TorresVani Xavier Jr Oliveira
Nov 6, 2015·Malaria Journal·Adriana Farias SilvaVani Xavier Oliveira
Feb 10, 2016·Journal of Peptide Science : an Official Publication of the European Peptide Society·Marcelo Der Torossian TorresVani Xavier Oliveira Junior
Apr 29, 2016·Oxidative Medicine and Cellular Longevity·Andreza Cândido MatiasGiselle Cerchiaro
May 12, 2016·Protein and Peptide Letters·Yashad DongolGopi Aryal
May 14, 2016·Biomacromolecules·Hadas Hecht, Simcha Srebnik
Dec 3, 2016·European Journal of Medicinal Chemistry·Cibele Nicolaski PedronVani Xavier Oliveira
May 22, 2017·Acta Biomaterialia·DaeYong LeeYeu-Chun Kim
Aug 11, 2017·Journal of Peptide Science : an Official Publication of the European Peptide Society·Marcelo Der Torossian TorresVani Xavier Oliveira

❮ Previous
Next ❯

Citations

Aug 10, 2019·Critical Reviews in Clinical Laboratory Sciences·Adriana Barreto-SantamaríaHernando Curtidor
Oct 24, 2019·Journal of Peptide Science : an Official Publication of the European Peptide Society·Isabel Cristina Chica AcevedoVani Xavier Oliveira
Nov 30, 2019·Chemical Communications : Chem Comm·Marcelo Der Torossian Torres, Cesar de la Fuente-Nunez
Oct 3, 2020·Bioengineering & Translational Medicine·Marcelo D T TorresCesar de la Fuente-Nunez
Jan 15, 2021·Journal of Peptide Science : an Official Publication of the European Peptide Society·Cibele Nicolaski PedronVani Xavier Oliveira Junior
Dec 5, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Sepideh MirzaeiManoj Garg
Nov 28, 2020·Chembiochem : a European Journal of Chemical Biology·Jessica A I MullerDavid J Craik
Jan 7, 2019·Journal of Molecular Biology·Marcelo D T TorresCesar de la Fuente-Nunez
May 15, 2021·Biotechnology Advances·Julien GiribaldiChristina I Schroeder
Jun 6, 2021·Journal of Colloid and Interface Science·Danubia Batista MartinsMarcia Perez Dos Santos Cabrera

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
AFM
ELISA
Facs

Software Mentioned

heliquest
Gwyddion

Related Concepts