Natural assembly of platelet lysate-loaded nanocarriers into enriched 3D hydrogels for cartilage regeneration

Acta Biomaterialia
Vítor E SantoR L Reis

Abstract

The role of Platelet Lysates (PLs) as a source of growth factors (GFs) and as main element of three-dimensional (3D) hydrogels has been previously described. However, the resulting hydrogels usually suffer from high degree of contraction, limiting their usefulness. This work describes the development of a stable biomimetic 3D hydrogel structure based on PLs, through the spontaneous assembling of a high concentration of chitosan-chondroitin sulfate nanoparticles (CH/CS NPs) with PLs loaded by adsorption. The interactions between the NPs and the lysates resemble the ones observed in the extracellular matrix (ECM) native environment between glycosaminoglycans and ECM proteins. In vitro release studies were carried out focusing on the quantification of PDGF-BB and TGF-β1 GFs. Human adipose derived stem cells (hASCs) were entrapped in these 3D hydrogels and cultured in vitro under chondrogenic stimulus, in order to assess their potential use for cartilage regeneration. Histological, immunohistological and gene expression analysis demonstrated that the PL-assembled constructs entrapping hASCs exhibited results similar to the positive control (hASCS cultured in pellets), concerning the levels of collagen II expression and immunolocali...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 26, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Ranieri CanceddaRodolfo Quarto
Aug 5, 2003·Biomaterials·Enrico LucarelliPiero Picci
Mar 3, 2005·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Kevin J ButterfieldDouglas Adams
Mar 23, 2007·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Akishige HokugoYasuhiko Tabata
May 15, 2007·Advanced Drug Delivery Reviews·Soo-Hong Lee, Heungsoo Shin
Nov 3, 2007·Advanced Drug Delivery Reviews·Cindy Chung, Jason A Burdick
Nov 27, 2007·Journal of Tissue Engineering and Regenerative Medicine·J F Mano, R L Reis
Jun 12, 2008·Tissue Engineering. Part B, Reviews·Tamer A E AhmedMax Hincke
Oct 10, 2008·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·C CsakiM Shakibaei
Oct 22, 2008·Journal of Tissue Engineering and Regenerative Medicine·S H ZakyM Mastrogiacomo
Nov 6, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hye-Joung Kim, Gun-Il Im
Dec 30, 2008·Journal of Tissue Engineering and Regenerative Medicine·T RadaM E Gomes
Feb 17, 2009·Tissue Engineering. Part C, Methods·Allan MishraChristopher R Jacobs
Jan 1, 2008·International Journal of Nanomedicine·Fabrizio Gelain
May 22, 2009·Nature Materials·Elsie S PlaceMolly M Stevens
Sep 5, 2009·Journal of Cellular Biochemistry·Adriano SpreaficoAnnalisa Santucci
Oct 8, 2009·American Journal of Rhinology & Allergy·August SageDeborah Watson
Nov 10, 2009·Cell and Tissue Research·Klaus von der MarkPatrik Schmuki
Dec 5, 2009·Birth Defects Research. Part C, Embryo Today : Reviews·Brent E BobickRocky S Tuan
May 25, 2010·Biomaterials·Fa-Ming ChenZhi-Fen Wu
Jul 2, 2010·Advanced Materials·Soheil Boddohi, Matt J Kipper
Oct 1, 2010·Advanced Materials·Prakriti Tayalia, David J Mooney
Feb 1, 2011·Clinics in Podiatric Medicine and Surgery·David J Soomekh
Mar 23, 2011·Chemical Reviews·Biji Balakrishnan, R Banerjee
Apr 16, 2011·Pharmaceutical Research·Nathaniel S HwangDaniel G Anderson
May 21, 2011·Tissue Engineering. Part B, Reviews·A Hari ReddiJosé A Andrades
Jun 12, 2012·Journal of Tissue Engineering and Regenerative Medicine·Vítor E SantoRui L Reis

❮ Previous
Next ❯

Citations

Dec 15, 2015·ACS Applied Materials & Interfaces·Flavia FontanaHélder A Santos
May 21, 2016·Biomacromolecules·Vítor E SantoManuela E Gomes
Feb 25, 2017·Biomaterials Science·Yon Jin ChuahDong-An Wang
May 9, 2018·Tissue Engineering. Part B, Reviews·Sara Catarina Nunes da Silva SantosJoão Filipe Colardelle da Luz Mano
Mar 30, 2017·Cold Spring Harbor Perspectives in Medicine·Johnny LamAntonios G Mikos
Sep 3, 2017·Journal of Biomedical Materials Research. Part a·Era JainScott A Sell
Jun 20, 2020·Biomedical Materials·Gülseren Irmak, Menemse Gumusderelioglu
Apr 18, 2018·Current Osteoporosis Reports·Mamatha M PillaiAmitava Bhattacharyya
Sep 29, 2020·Biomedical Materials·Gülseren Irmak, Menemşe Gümüşderelioğlu
Dec 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Manuel Gomez-FloritManuela E Gomes
May 10, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Gülseren Irmak, Menemşe Gümüşderelioğlu
Aug 18, 2020·ACS Applied Materials & Interfaces·Zijie XiahouJingbo Yin
Apr 9, 2018·ACS Biomaterials Science & Engineering·Ujjal Didar Singh Sekhon, Anirban Sen Gupta
May 3, 2020·Current Stem Cell Research & Therapy·Lang WangJingang Xiao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.