Natural autoantibodies against glomerular basement membrane exist in normal human sera

Kidney International
Zhao CuiM-h Zhao

Abstract

Autoimmunity to glomerular basement membrane (GBM) could induce Goodpasture disease, and natural autoantibodies against GBM in the sera of normal individuals were not reported. The aim of the study was to identify and characterize natural autoantibodies against GBM in normal human sera. Natural anti-GBM autoantibodies were purified from the sera of five healthy persons by affinity chromatography, using purified bovine alpha(IV)non-collagenous (NC1) as solid-phase ligands. Antigen specificity, immunoglobulin G (IgG) subclasses, and antibody avidity of the natural autoantibodies were investigated by enzyme-linked immunosorbant assay (ELISA), Western-blot analysis, indirect immunofluorescence, and antigen-inhibition ELISA, and compared with those of 32 patients with anti-GBM disease. Natural anti-GBM autoantibodies could be purified from IgG fractions of all the five persons, with an average amount of 0.5% of total IgG fractions. Antigen specificity of the natural autoantibodies was identified by blotting to human alpha(IV)NC1, reactivity to recombinant alpha3(IV)NC1, and linear staining along the GBM of normal kidney sections. Titers of the natural autoantibodies were much lower than those of patients (1:60.6 vs 1:993.6, P<0.001)...Continue Reading

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