Natural competence for DNA transformation in Helicobacter pylori: identification and genetic characterization of the comB locus

Molecular Microbiology
D HofreuterRainer Haas

Abstract

The gram-negative bacterial pathogen Helicobacter pylori, an important aetiological agent of gastroduodenal disease in humans, belongs to a group of bacterial species displaying competence for genetic transformation. Here, we describe the comB gene locus of H. pylori involved in DNA transformation competence. It consists of a cluster of four tandemly arranged genes with partially overlapping open reading frames, orf2, comB1, comB2 and comB3, constituting a single transcriptional unit. Orf2 encodes a 37-amino-acid peptide carrying a signal sequence, whereas comB1, comB2 and comB3 produce 29 kDa, 38 kDa and 42 kDa proteins, respectively, as demonstrated by immunoblotting with specific antisera. For Orf2 and ComB1, no homologous proteins were identified in the database. For ComB3, the best homologies were found with TraS/TraB from the Pseudomonas aeruginosa conjugative plasmid RP1 and TrbI of plasmid RP4, VirB10 from the Ti plasmid of Agrobacterium tumefaciens and PtlG, a protein involved in secretion of pertussis toxin of Bordetella pertussis. Defined transposon knock-out mutants in individual comB genes resulted in transformation-defective phenotypes ranging from a 90% reduction to a complete loss of the natural transformation e...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D H Figurski, D R Helinski
Oct 1, 1992·Molecular Microbiology·H LeyingR Haas
Oct 17, 1991·The New England Journal of Medicine·J ParsonnetR K Sibley
Oct 17, 1991·The New England Journal of Medicine·A NomuraM J Blaser
Sep 1, 1991·Microbiological Reviews·D Dubnau
May 24, 1991·Science·A LupasJ Stock
Feb 1, 1990·The Journal of Infectious Diseases·P Nedenskov-SørensenK Bøvre
Feb 1, 1990·Journal of Bacteriology·Y Wang, D E Taylor
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M L Pifer, H O Smith
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Shine, L Dalgarno
May 14, 1969·Journal of Molecular Biology·H W Boyer, D Roulland-Dussoix
Nov 1, 1966·Journal of Bacteriology·P F Sparling
Sep 20, 1995·Molecular & General Genetics : MGG·W SchmittR Haas
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·L S HåvarsteinD A Morrison
Sep 1, 1994·Microbiological Reviews·B Dreiseikelmann
Sep 1, 1994·Microbiological Reviews·M G Lorenz, W Wackernagel
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A A WeissD L Burns
Feb 1, 1996·Trends in Microbiology·S C WinansP J Christie
Apr 1, 1996·Trends in Genetics : TIG·J M Solomon, A D Grossman

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Citations

May 26, 2004·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ian M CarrollNiyaz Ahmed
Aug 29, 2000·Research in Microbiology·D HofreuterR Haas
Jan 21, 2000·FEMS Immunology and Medical Microbiology·L C SmeetsJ G Kusters
Apr 15, 2004·Nature Reviews. Microbiology·Inês Chen, David Dubnau
Sep 17, 2004·Nature Reviews. Microbiology·Denise M MonackStanley Falkow
Mar 16, 2011·Genome Biology and Evolution·Isabelle C ArnoldAnne Müller
Nov 15, 2006·Infection and Immunity·David N BaldwinNina R Salama
Jul 19, 2000·Infection and Immunity·D J BaconP Guerry
Jun 27, 2000·Journal of Bacteriology·L C SmeetsJ G Kusters
Sep 2, 2003·Journal of Bacteriology·Rebecca S WiesnerVictor J DiRita
Sep 18, 2004·Journal of Bacteriology·Joseph C LarsenPatricia Guerry
Nov 18, 2004·Journal of Bacteriology·Nina R SalamaStanley Falkow
Jan 20, 2004·Journal of Bacteriology·Wolfgang Fischer, Rainer Haas
Dec 1, 2009·Microbiology and Molecular Biology Reviews : MMBR·Cristina E Alvarez-Martinez, Peter J Christie
Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Jeremy J GilbreathDavid R Hendrixson
Aug 11, 2012·BMC Microbiology·Rangel C SouzaMarisa F Nicolás
Oct 28, 2008·BMC Gastroenterology·Zhiyu ZhangHong Lu
Jan 7, 2005·Annals of Clinical Microbiology and Antimicrobials·Niyaz Ahmed, Leonardo A Sechi
Mar 10, 2011·PloS One·Edgar Eduardo Lara-RamírezMario A Rodríguez-Pérez
Oct 3, 2012·PloS One·Stefanie RohrerRainer Haas
Nov 2, 2001·Biochemical and Biophysical Research Communications·K C ChangJ T Wang
Feb 12, 2014·Journal of Gastroenterology·Esther Fernandez-Gonzalez, Steffen Backert
Oct 24, 1998·Molecular Microbiology·C C McGowanM J Blaser
Jun 6, 2003·Molecular Microbiology·Catherine C McGowanMartin J Blaser
May 30, 2006·Journal of Molecular Biology·Stephen HareGabriel Waksman
Jul 19, 2003·International Journal of Medical Microbiology : IJMM·Dirk HofreuterRainer Haas
Apr 26, 2008·Journal of Medical Microbiology·Yoshio Yamaoka
Apr 28, 2009·FEMS Microbiology Reviews·Ole Herman AmburTone Tønjum
Oct 10, 2006·FEMS Microbiology Reviews·Raúl Fernández-LópezFernando de la Cruz

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