Natural Cytotoxicity Receptors in Decidua Natural Killer Cells of Term Normal Pregnancy

Journal of Pregnancy
Hideki TakahashiFumihisa Chishima

Abstract

To investigate the changes in the maternal immune system at term pregnancy, we studied the expression of natural cytotoxicity receptors (NCRs) and the cytokine production of NK cells in term placenta decidua and peripheral blood. Term decidua and peripheral blood were taken from patients undergoing elective cesarean section. The lymphocytes were separated using density gradient centrifugation (DGC) from peripheral blood and were separated from decidua using DGC after enzyme digestion. These cells were stained with FITC anti-CD56 and Per-CP anti-CD3 monoclonal antibodies, and the NCRs were stained with PE-conjugated anti-NKG2D, NKp46, NKp30, and NKp44 monoclonal antibodies. Cytokines, including IFN-γ, TNF-α, IL-10, and TGF-β, were stained and then analyzed by flow cytometry. There were fewer cells positive for NKG2D, NKp46, and NKp30 among CD56+CD3- cells in deciduas than in peripheral blood, but the percentages of NKp44-positive cells in CD56+CD3- lymphocytes in deciduas tended to be higher. The decreased expression of some NCRs in deciduas may be related to decreased cytotoxicity at term pregnancy, but the increased expression of NKp44 may affect the increased cytokine production in the decidua. Similarly, the expression of NC...Continue Reading

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Citations

May 8, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ricardo de Mendonça VieiraTamara Tilburgs
May 30, 2020·Cellular & Molecular Immunology·Derek MillerNardhy Gomez-Lopez

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Methods Mentioned

BETA
cesarean section
flow cytometry
Flow

Software Mentioned

GraphPad Prism
FlowJo

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