Natural homolog of tRNA synthetase editing domain rescues conditional lethality caused by mistranslation.

The Journal of Biological Chemistry
Yeeting E ChongP Schimmel

Abstract

AlaXp is a widely distributed (from bacteria to humans) genome-encoded homolog of the editing domain of alanyl-tRNA synthetases. Editing repairs the confusion of serine and glycine for alanine through clearance of mischarged (with Ser or Gly) tRNA(Ala). Because genome-encoded fragments of editing domains of other synthetases are scarce, the AlaXp redundancy of the editing domain of alanyl-tRNA synthetase is thought to reflect an unusual sensitivity of cells to mistranslation at codons for Ala. Indeed, a small defect in the editing activity of alanyl-tRNA synthetase is causally linked to neurodegeneration in the mouse. Although limited earlier studies demonstrated that AlaXp deacylated mischarged tRNA(Ala) in vitro, the significance of this activity in vivo has not been clear. Here we describe a bacterial system specifically designed to investigate activity of AlaXp in vivo. Serine toxicity, experienced by a strain harboring an editing-defective alanyl-tRNA synthetase, was rescued by an AlaXp-encoding transgene. Rescue was dependent on amino acid residues in AlaXp that are needed for its in vitro catalytic activity. Thus, the editing activity per se of AlaXp was essential for suppressing mistranslation. The results support the i...Continue Reading

References

Sep 1, 1992·Microbiological Reviews·H Jakubowski, E Goldman
Jul 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M Yarus
Apr 27, 2000·Trends in Biochemical Sciences·P Schimmel, L Ribas De Pouplana
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·P J Beuning, K Musier-Forsyth
Sep 24, 2002·The Journal of Biological Chemistry·Leslie A NanglePaul Schimmel
Oct 8, 2003·The Journal of Biological Chemistry·Fai-Chu WongKarin Musier-Forsyth
Dec 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ivan AhelDieter Söll
Jul 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Dragana KorencicDieter Söll
Jan 14, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jamie M BacherPaul R Schimmel
Aug 10, 2005·The Journal of Biological Chemistry·Songon An, Karin Musier-Forsyth
Aug 10, 2005·Proceedings of the National Academy of Sciences of the United States of America·Masaaki SokabeIsao Tanaka
Oct 21, 2006·Chemistry & Biology·Leslie A NanglePaul Schimmel
Mar 1, 2007·Acta Crystallographica. Section D, Biological Crystallography·Ryuya Fukunaga, Shigeyuki Yokoyama
Jan 4, 2008·Nature·Kirk BeebePaul Schimmel

❮ Previous
Next ❯

Citations

Mar 31, 2012·The Journal of Physical Chemistry. B·Sandeep KumarKarin Musier-Forsyth
Nov 29, 2012·The Journal of Physical Chemistry. B·Sandeep KumarKarin Musier-Forsyth
Nov 21, 2008·Proceedings of the National Academy of Sciences of the United States of America·Michal T BonieckiSusan A Martinis
Jun 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Masaaki SokabeIsao Tanaka
Feb 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Mir Hussain NawazPaul Schimmel
Feb 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jiqiang LingMiljan Simonović
Feb 12, 2011·The Journal of Biological Chemistry·My-Nuong VoPaul Schimmel
Jun 24, 2009·The Journal of Biological Chemistry·John M ZaborskeRonald C Wek
Jul 20, 2011·The Journal of Biological Chemistry·Byung Ran SoKarin Musier-Forsyth
Apr 1, 2009·The Journal of Biological Chemistry·Sandra WydauSylvain Blanquet
Sep 21, 2011·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Paul Schimmel
Mar 3, 2010·Annual Review of Biophysics·Jack A Dunkle, Jamie H D Cate
Apr 22, 2009·Annual Review of Microbiology·Jiqiang LingMichael Ibba
Oct 16, 2013·Molecular and Cellular Biology·Igor CestariKenneth Stuart
Sep 16, 2014·FEBS Letters·Adil MoghalMichael Ibba
Apr 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Ziwei LiuKarin Musier-Forsyth
Nov 26, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ye LiuSusan L Ackerman
Dec 14, 2011·Current Opinion in Structural Biology·Min Guo, Paul Schimmel
Nov 19, 2011·Wiley Interdisciplinary Reviews. RNA·Hieronim Jakubowski
Apr 8, 2014·Wiley Interdisciplinary Reviews. RNA·Yan Ling Joy PangSusan A Martinis
Dec 6, 2012·The Biochemical Journal·Liang-Liang RuanEn-Duo Wang
Mar 23, 2015·FEMS Microbiology Reviews·Jennifer Shepherd, Michael Ibba
Jan 24, 2019·The Journal of Biological Chemistry·Christopher S Francklyn, Patrick Mullen
Dec 22, 2019·The Journal of Biological Chemistry·Nien-Ching HanMichael Ibba
Mar 3, 2020·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Nozomu Mori
Sep 10, 2020·RNA Biology·Matthew D Berg, Christopher J Brandl
Mar 1, 2015·The Journal of Biological Chemistry·Eva Maria NovoaLluís Ribas de Pouplana
Dec 16, 2020·RNA Biology·Han ZhangLitao Sun
Apr 1, 2021·Proceedings of the National Academy of Sciences of the United States of America·Surya D AggarwalN Luisa Hiller
Nov 1, 2012·EcoSal Plus·Richard Giegé, Mathias Springer
May 26, 2016·EcoSal Plus·Richard Giegé, Mathias Springer

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Masaaki SokabeIsao Tanaka
Proceedings of the National Academy of Sciences of the United States of America
Mir Hussain NawazP Schimmel
Proceedings of the National Academy of Sciences of the United States of America
Masaaki SokabeIsao Tanaka
Proceedings of the National Academy of Sciences of the United States of America
Masahiro NaganumaShigeyuki Yokoyama
© 2022 Meta ULC. All rights reserved