Natural human isolates of Staphylococcus aureus selected for high production of proteases and alpha-hemolysin are sigmaB deficient

International Journal of Medical Microbiology : IJMM
Anna Karlsson-KanthJan Oscarsson

Abstract

It has been reported that high production of proteases and alpha-hemolysin in the prototype Staphylococcus aureus strain 8325-4 was associated with its sigmaB deficiency. Here we analyzed one fresh clinical isolate (KS26) and two ancient human isolates (Wood46 and V8) selected for high production of proteases and alpha-hemolysin. All three strains lacked yellow pigment and showed a low level of expression of sigB-dependent promoters, indicating sigmaB deficiency. Nucleotide sequencing of the sigB operon revealed that KS26 and Wood46 had stop codons in rsbU and sigB, respectively, while V8 had an insertion of an IS element in rsbU. Complementation experiments with sigB on a plasmid reduced expression of proteases and alpha-hemolysin dramatically, indicating that the high production of these exoproteins was associated with sigmaB deficiency. Although sigmaB-deficient strains show attenuated virulence in some animal models, our results indicate that such strains can cause infection in humans.

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Citations

Mar 6, 2013·Antimicrobial Agents and Chemotherapy·Joshua B ParsonsCharles O Rock
Jun 22, 2006·Journal of Bacteriology·Sünje Johanna PampHanne Ingmer
Feb 6, 2007·Journal of Bacteriology·Jaione ValleIñigo Lasa
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Sep 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marijo ParcinaIsabelle Bekeredjian-Ding
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Aug 28, 2021·International Journal of Molecular Sciences·Katharina MayerTom Grunert
Aug 4, 2021·Annual Review of Microbiology·Lisa Bowman, Tracy Palmer

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