Natural Isotopic Abundance 13 C and 15 N Multidimensional Solid-State NMR Enabled by Dynamic Nuclear Polarization

The Journal of Physical Chemistry Letters
Adam N SmithGaël De Paëpe

Abstract

Dynamic nuclear polarization (DNP) has made feasible solid-state NMR experiments that were previously thought impractical due to sensitivity limitations. One such class of experiments is the structural characterization of organic and biological samples at natural isotopic abundance (NA). Herein, we describe the many advantages of DNP-enabled ssNMR at NA, including the extraction of long-range distance constraints using dipolar recoupling pulse sequences without the deleterious effects of dipolar truncation. In addition to the theoretical underpinnings in the analysis of these types of experiments, numerous applications of DNP-enabled ssNMR at NA are discussed.

References

Nov 22, 1993·Physical Review Letters·L R BecerraR G Griffin
Dec 1, 2000·Journal of Magnetic Resonance·M BakN C Nielsen
Sep 5, 2002·Journal of the American Chemical Society·Christopher P JaroniecRobert G Griffin
Jul 10, 2003·Journal of the American Chemical Society·James L SudmeierWilliam W Bachovchin
Dec 13, 2005·Journal of Magnetic Resonance·Mikhail Veshtort, Robert G Griffin
Feb 9, 2006·Journal of the American Chemical Society·Gaël De PaëpeRobert G Griffin
Jul 11, 2006·The Journal of Chemical Physics·Per Eugen KristiansenMalcolm H Levitt
Aug 31, 2006·Journal of the American Chemical Society·Changsik SongRobert G Griffin
Jan 25, 2007·Journal of the American Chemical Society·Józef R LewandowskiRobert G Griffin
Feb 3, 2007·The Journal of Pharmacy and Pharmacology·Robin K Harris
Feb 13, 2008·The Journal of Chemical Physics·Kan-Nian HuRobert G Griffin
Jan 7, 2009·The Journal of Chemical Physics·Gaël De PaëpeRobert G Griffin
Aug 20, 2009·Journal of Biomolecular NMR·Lenka SkrisovskaFrédéric H-T Allain
Jan 1, 2010·Journal of the American Chemical Society·Ivano BertiniClaudio Luchinat
Feb 9, 2010·Journal of the American Chemical Society·Elodie SalagerLyndon Emsley
Sep 14, 2010·Journal of the American Chemical Society·Anne LesageLyndon Emsley
Oct 14, 2010·Angewandte Chemie·Marie RenaultMarc Baldus
Mar 10, 2011·The Journal of Chemical Physics·Gaël De PaëpeRobert G Griffin
Mar 16, 2011·Journal of the American Chemical Society·Antoine LoquetAdam Lange
Mar 13, 2012·Annual Review of Physical Chemistry·Gaël De Paëpe
Sep 25, 2012·Journal of Magnetic Resonance·Frederic Mentink-VigierAkiva Feintuch
Feb 6, 2013·Journal of the American Chemical Society·Frédéric BlancClare P Grey
Mar 23, 2013·Accounts of Chemical Research·Aaron J RossiniLyndon Emsley
Jan 15, 2014·Journal of the American Chemical Society·Aaron J RossiniLyndon Emsley
Aug 6, 2014·Journal of Magnetic Resonance·Zdeněk TošnerThomas Vosegaard
Dec 17, 2014·Angewandte Chemie·Adam N SmithJoanna R Long
Feb 11, 2015·Chemistry : a European Journal·Carlos Fernández-de-AlbaGaël De Paëpe
Mar 18, 2015·Solid State Nuclear Magnetic Resonance·Daniel LeeGaël De Paëpe
May 15, 2015·Protein Science : a Publication of the Protein Society·Byungsu KwonMei Hong
Aug 1, 2015·The Journal of Physical Chemistry. B·Maxim A VoinovAlex I Smirnov
Aug 4, 2015·Physical Chemistry Chemical Physics : PCCP·Frédéric Mentink-VigierGaël De Paëpe
Aug 16, 2015·The Journal of Physical Chemistry Letters·Chandan SinghNeeraj Sinha

❮ Previous
Next ❯

Citations

Jan 31, 2020·Physical Chemistry Chemical Physics : PCCP·Frédéric Mentink-Vigier
May 8, 2020·Biochemical Society Transactions·Arnab ChakrabortyTuo Wang
May 13, 2020·Chemistry : a European Journal·Sucharita Mandal, Snorri Th Sigurdsson
May 22, 2020·Frontiers in Chemistry·Djalal TracheM Hazwan Hussin
Aug 8, 2020·Magnetic Resonance in Chemistry : MRC·Nidhi TiwariNeeraj Sinha
Mar 13, 2020·Chemical Science·Akshay KumarGaël De Paëpe

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.