PMID: 8979153Dec 1, 1996Paper

Natural killer and lectin-dependent cytotoxic activities of Kurloff cells: target cell selectivity, conjugate formation, and Ca++ dependency

Inflammation
N PouliotM Rola-Pleszczynski

Abstract

Kurloff cells may represent a major component of NK cell activity in the guinea pig. We have pursued to characterize the mechanism of their action. Using murine target cells, we found Kurloff cell cytotoxicity to be selective for the NK-sensitive YAC-1 target cell, with minimal activity against the NK-resistant P815 target cell. In the presence of PHA, but not ConA, cytotoxicity was markedly augmented against both YAC-1 and P815. While effector-target conjugate formation was observed with YAC-1 cells but not P815 cells in control cultures, it was augmented with both target cell types in cultures with PHA. Pretreatment alone with PHA was ineffective, however. NK cell activity of Kurloff cells was dependent on extracellular Ca++ and entry of Ca++ into the effector cells, as demonstrated by abrogation of cytotoxicity when extracellular Ca++ was chelated with EDTA or EGTA, or following treatment with the Ca++ channel blockers verapamil and diltiazem. Furthermore, inhibition of PKC by H7 resulted in significant reduction of Kurloff cell-mediated NK activity, while pretreatment of effector cells with the PKC activator TPA enhanced NK activity. Kurloff cells could also be stimulated to produce serine esterases by contact with target c...Continue Reading

References

Mar 1, 1977·Transplantation·B BonavidaA Saxon
Jan 1, 1990·Journal of Leukocyte Biology·E A AtkinsonA H Greenberg
Nov 15, 1987·Biochemical Pharmacology·J NgM Jondal
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J K BurkhardtY Argon
Jan 1, 1971·International Archives of Allergy and Applied Immunology·A H MarshallP A Revell
Jan 1, 1973·International Archives of Allergy and Applied Immunology·A B Wilson, R R Coombs
Jan 1, 1971·Scandinavian Journal of Haematology·U Ernström, G Sandberg
Jan 1, 1972·Experimental Cell Research·A BoydeP Veselý
Jan 1, 1974·International Archives of Allergy and Applied Immunology·F WisloffT E Michaelsen
Jan 1, 1973·Annual Review of Biochemistry·H Lis, N Sharon
Jan 1, 1971·International Archives of Allergy and Applied Immunology·A B Wilson, R R Coombs
Mar 1, 1971·American Journal of Clinical Pathology·L T YamW H Crosby
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T FreyH M McConnell

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Citations

Oct 7, 2000·Journal of Gastroenterology and Hepatology·T TsukishiroA Watanabe
Mar 19, 2003·International Journal of Experimental Pathology·J A TurtonT C Williams

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