Natural killer cell activation and modulation of chemokine receptor profile in vitro by an extract from the cyanophyta Aphanizomenon flos-aquae

Journal of Medicinal Food
Aaron N HartGitte S Jensen

Abstract

The present research was designed to study the effects of an extract from the edible cyanophyta Aphanizomenon flos-aquae on human natural killer (NK) cells. We have previously shown, using a double-blind randomized placebo-controlled crossover design, that ingestion of 1.5 g of dried whole A. flos-aquae resulted in a transient reduction in peripheral blood NK cells in 21 healthy human volunteers, suggesting increased NK cell homing into tissue. We have now identified an extract from A. flos-aquae (AFAe) that directly activates NK cells in vitro and modulates the chemokine receptor profile. NK cell activation was evaluated by expression of CD25 and CD69 on CD3-CD56+ cells after 18 hours. Changes in CXCR3 and CXCR4 chemokine receptor expression after 5-60 minutes were evaluated by immunostaining and flow cytometry. AFAe induced the expression of CD69 on CD3-CD56+ NK cells, induced CD25 expression on 25% of these cells, and acted in synergy with interleukin 2. NK cells enriched by RosetteSep (StemCell Technologies Inc., Vancouver, BC, Canada) were not activated by AFAe, indicating that the NK activation was dependent on other cells such as monocytes. The low-molecular-weight fraction <5,000 of AFAe was responsible for the most rob...Continue Reading

References

Jun 11, 1999·The Journal of Biological Chemistry·R SchwandnerC J Kirschning
Dec 23, 1999·Current Opinion in Plant Biology·G I McFadden
Jan 29, 2000·Immunology and Cell Biology·T Matsuzaki, J Chin
May 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·J J CampbellE C Butcher
Feb 5, 2002·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·N Pugh, D S Pasco
Apr 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Veit HornungGunther Hartmann
Mar 23, 2004·Annual Review of Immunology·Wayne M YokoyamaAnthony R French
Sep 15, 2004·FEMS Immunology and Medical Microbiology·Ge LiuKen Alibek
Jan 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yoshiya NakaoMisako Matsumoto
Feb 16, 2006·Current Allergy and Asthma Reports·David Proud
Aug 17, 2006·Current Topics in Microbiology and Immunology·D M E BowdishR E W Hancock

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Citations

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