Natural killer cells contribute to mitochondrial dysfunction in response to placental ischemia in reduced uterine perfusion pressure rats

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Venkata Ramana VakaBabbette LaMarca

Abstract

Preeclampsia (PE) is characterized by new-onset hypertension during pregnancy and is associated with immune activation and placental oxidative stress. Mitochondrial dysfunction is a major source of oxidative stress and may play a role in the pathology of PE. We (Vaka VR, et al. Hypertension 72: 703-711, 2018. doi: 10.1161/HYPERTENSIONAHA.118.11290 .) have previously shown that placental ischemia is associated with mitochondrial oxidative stress in the reduced uterine perfusion pressure (RUPP) model of PE. Furthermore, we have also shown that placental ischemia induces natural killer (NK) cell activation in RUPP. Thus, we hypothesize that NK cell depletion could improve mitochondrial function associated with hypertension in the RUPP rat model of PE. Pregnant Sprague-Dawley rats were divided into three groups: normal pregnant (NP), RUPP, and RUPP+NK cell depletion rats (RUPP+NKD). On gestational day (GD)14, RUPP surgery was performed, and NK cells were depleted by administering anti-asialo GM1 antibodies (3.5 µg/100 µl ip) on GD15 and GD17. On GD19, mean arterial pressure (MAP) was measured, and placental mitochondria were isolated and used for mitochondrial assays. MAP was elevated in RUPP versus NP rats (119 ± 1 vs.104 ± 2 mmHg...Continue Reading

References

Mar 1, 1997·Research in Immunology·C M Eischen, P J Leibson
Dec 16, 1998·British Journal of Obstetrics and Gynaecology·J M MorrisC W Redman
Feb 6, 2003·Journal of Hypertension·Virginia M LeeLeong L Ng
Mar 8, 2003·Hypertension·James M RobertsUNKNOWN NHLBI Working Group on Research on Hypertension During Pregnancy
Aug 2, 2003·Seminars in Perinatology·Baha M SibaiUNKNOWN National Institute of Child Health and Human Development Maternal-Fetal Medicine Units Network
May 15, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Joey P Granger
Oct 6, 2009·Brain, Behavior, and Immunity·Khalil Karimi, Petra Clara Arck
Sep 6, 2011·Trends in Immunology·Paola VaccaMaria Cristina Mingari
Mar 1, 2012·American Journal of Reproductive Immunology : AJRI·Atsushi FukuiHideki Mizunuma
Dec 27, 2012·The World Allergy Organization Journal·Esra BirbenOmer Kalayci
Nov 2, 2014·Cell Death and Differentiation·G JacqueminD Martinvalet
Mar 5, 2015·International Journal of Molecular Sciences·Keiichi MatsubaraAkihiro Nawa
Oct 19, 2017·Clinical Science·Jamil ElfarraDenise C Cornelius

❮ Previous
Next ❯

Citations

Jul 11, 2020·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Mark W CunninghamBabbette LaMarca
Apr 23, 2020·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Olivia K TravisDenise C Cornelius
Nov 17, 2020·American Journal of Physiology. Renal Physiology·Evangeline DeerBabbette LaMarca
Apr 4, 2021·Antioxidants·Xiang-Qun Hu, Lubo Zhang
Apr 14, 2021·Journal of Leukocyte Biology·Derek MillerNardhy Gomez-Lopez

❮ Previous
Next ❯

Datasets Mentioned

BETA
GM-121334

Methods Mentioned

BETA
FACS

Software Mentioned

Oxygraph

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.