PMID: 2505079Mar 1, 1989Paper

Natural killer cells in inflammatory lesions and transplanted tumors in mouse skin

Nagoya Journal of Medical Science
L NaharP K Nakane

Abstract

The mode of natural killer (NK) cell migration to the sites of inflammation and transplanted tumors was investigated by using dry ice for physical irritation and 1-fluoro-2,4 dinitrobenzene (DNFB) for chemical irritation in mouse ear. In experiments with transplanted tumors, NK cell sensitive tumor cells (RL male 1) and insensitive tumor cells (p815) were transplanted into the ears of C3H and BALB/c mice, respectively. Employing a polyclonal rabbit antiserum against asialoGM1 (GA1), and a monoclonal rat antiserum against Thy-1 in an immunohistochemical double-staining technique, we enumerated the number of Thy-1-positive and asialoGM1-positive (Thy-1+ GA1+) cells and Thy-1-negative and asialoGM1-positive (Thy-1-GA1+) cells at various times of irritation. Following physical irritation, Thy-1-GA1+ cells (108.8 +/- 4.5/mm2 at 24 h and 71.2 +/- 3.8/mm2 at 48 h) were found in the epidermis, whereas Thy-1+GA1+ cells were not found. In delayed-type skin reaction by DNFB, Thy-1-GA1+ cells (87.1 +/- 5.8/mm2 at 24 h and 60.7 +/- 2.9/mm2 at 48 h) and Thy-1+GA1+ cells (26.4 +/- 3.6/mm2 at 24 h and 15.3 +/- 4.3/mm2 at 48 h) were found in the dermis. Since it was reported by previous investigators that Thy-1+GA1+ cells are NK cells, we assum...Continue Reading

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