Natural Killer Cells Response to IL-2 Stimulation Is Distinct between Ascites with the Presence or Absence of Malignant Cells in Ovarian Cancer Patients

International Journal of Molecular Sciences
Rodrigo Fernandes da SilvaFernando Guimarães

Abstract

Peritoneal ascites are a distinguishable feature of patients with advanced epithelial ovarian cancer (EOC). The presence of different lymphocyte subsets has been reported in EOC-associated ascites, which also can or not contain malignant cells. The goal of this study was to analyze the functional characteristics of natural killer (NK) cells from EOC-associated ascites in terms of their expression of activating receptors and ascites' contents of lymphocyte subtypes, cytokine profile and presence of EOC cells. NK cell function was evaluated by the expression of the degranulation marker CD107a in resting and interleukin (IL)-2 stimulated NK cells from ascites and blood. Degranulation of NK cells from EOC cell-free ascites was significantly (p< 0.05) higher than all the other groups, either in their resting state or after IL-2 stimulation, suggesting a previous local stimulation. In contrast, treatment with IL-2 had no effect on NK cells from ascites with EOC cells. The amount of regulatory T cells was significantly higher in ascites with EOC cells compared to EOC cell-free ascites. Ascites with EOC cells also had higher levels of tumor necrosis factor (TNF)-α, suggesting inflammation related to the malignancy. In conclusion, the f...Continue Reading

References

Aug 15, 1975·International Journal of Cancer. Journal International Du Cancer·R B HerbermanD H Lavrin
Aug 15, 1988·International Journal of Cancer. Journal International Du Cancer·E LotzováJ T Wharton
Jan 1, 1986·Comparative Immunology, Microbiology and Infectious Diseases·E LotzováJ M Bowen
Jan 1, 1983·Cancer Metastasis Reviews·M Introna, A Mantovani
Jan 1, 1996·The British Journal of Surgery·S L ParsonsR J Steele
Mar 13, 2001·Annual Review of Immunology·J V Ravetch, S Bolland
Mar 22, 2002·Nature Reviews. Immunology·A Cerwenka, L L Lanier
Aug 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Udaya K LiyanageDavid C Linehan
Jan 17, 2003·The New England Journal of Medicine·Lin ZhangGeorge Coukos
Dec 20, 2003·The EMBO Journal·Lorenzo Moretta, Alessandro Moretta
Mar 24, 2005·Nature Immunology·Harald von Boehmer
May 3, 2005·Nature Reviews. Cancer·Honami Naora, Denise J Montell
Sep 30, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Susan K LutgendorfDavid M Lubaroff
Oct 19, 2005·The Journal of Experimental Medicine·François GhiringhelliLaurence Zitvogel
Nov 25, 2005·American Journal of Reproductive Immunology : AJRI·Brian BarnettTyler J Curiel
Jan 19, 2006·Blood·Dong-Mei ZhaoEthan M Shevach
Jan 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mark J SmythYoshihiro Hayakawa
Jul 5, 2006·The Journal of Experimental Medicine·Weihong LiuJeffrey A Bluestone
Aug 9, 2006·Seminars in Cancer Biology·Tal I ArnonOfer Mandelboim
Apr 25, 2008·Current Protocols in Immunology·Megan A Cooper, Michael A Caligiuri
Sep 2, 2008·Immunological Reviews·Eric O Long
Oct 29, 2008·Acta Pharmacologica Sinica·Xia Wang, Yong Lin
Feb 4, 2009·The Journal of Experimental Medicine·Randall H FriedlineCynthia A Chambers
Oct 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mattias CarlstenKarl-Johan Malmberg
Dec 25, 2009·Methods in Molecular Biology·Yenan T BrycesonHans-Gustaf Ljunggren
Feb 11, 2010·Gynecologic Oncology·Refika YigitRuurd Torensma
Aug 2, 2011·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Keisuke OtsuboToshio Miyawaki
Aug 31, 2011·Cellular and Molecular Life Sciences : CMLS·Tomonori KaifuMyriam Baratin
Nov 2, 2011·Gynecologic Oncology·Wei-Ting HwangGeorge Coukos
Apr 16, 2013·Critical Reviews in Immunology·Bridget CharbonneauMelissa S Derycke
Apr 15, 2014·Clinical Immunology : the Official Journal of the Clinical Immunology Society·A BoldtU Sack
Jun 3, 2014·Molecular Cancer·Mildred FelderManish S Patankar
Jul 19, 2014·Cancer Discovery·Arabella YoungMark J Smyth

❮ Previous
Next ❯

Citations

Mar 14, 2019·Journal of Cellular Physiology·Saeid ShahrabiElham Rajaei
Sep 21, 2017·Protein & Cell·Boyi ZhangYu Sun
Sep 4, 2019·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·Cid Almeida de LimaRosekeila Simões Nomelini
Feb 23, 2019·International Journal of Molecular Sciences·Marco GreppiEmanuela Marcenaro

❮ Previous
Next ❯

Methods Mentioned

BETA
surgical resection
Assay
flow cytometry

Software Mentioned

FACSuite
Tree Star
FlowJo

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.