Natural killer cells target HCV core proteins during the innate immune response in HCV transgenic mice

Journal of Medical Virology
Kenichi SatohMichinori Kohara

Abstract

The mechanism of the innate immune response to hepatitis C virus (HCV) has not been fully elucidated, largely due to the lack of an appropriate model. We used HCV transgenic (Tg) mice, which express core, E1, E2, and NS2 proteins regulated by the Cre/loxP switching expression system, to examine the innate immune response to HCV structural proteins. Twelve hours after HCV transgene expression, HCV core protein levels in Tg mouse livers were 15-47 pg/mg. In contrast, in Tg mice with a depletion of natural killer (NK) cells, we observed much higher levels of HCV core proteins (1,597 pg/ml). Cre-mediated genomic DNA recombination efficiency in the HCV-Tg mice was strongly observed in NK cell-depleted mice between 0.5 and 1 day as compared to non-treated mice. These data indicated that NK cells participate in the elimination of core-expressing hepatocytes in the innate immune responses during the acute phase of HCV infection.

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Citations

Feb 18, 2011·Journal of Gastroenterology·Angela Dolganiuc, Gyongyi Szabo
Apr 23, 2011·Experimental Animals·Kiminori Kimura, Michinori Kohara
Jan 1, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yan LiuSteven Dooley
Aug 8, 2021·Cells·Niloofar Khoshdel-RadMassoud Vosough

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