Natural Microbial Community Compositions Compared by a Back-Propagating Neural Network and Cluster Analysis of 5S rRNA.

Applied and Environmental Microbiology
Peter A NobleM Fletcher

Abstract

The community compositions of free-living and particle-associated bacteria in the Chesapeake Bay estuary were analyzed by comparing banding patterns of stable low-molecular-weight RNA (SLMW RNA) which include 5S rRNA and tRNA molecules. By analyzing images of autoradiographs of SLMW RNAs on polyacrylamide gels, band intensities of 5S rRNA were converted to binary format for transmission to a back-propagating neural network (NN). The NN was trained to relate binary input to sample stations, collection times, positions in the water column, and sample types (e.g., particle-associated versus free-living communities). Dendrograms produced by using Euclidean distance and average and Ward's linkage methods on data of three independently trained NNs yielded the following results. (i) Community compositions of Chesapeake Bay water samples varied both seasonally and spatially. (ii) Although there was no difference in the compositions of free-living and particle-associated bacteria in the summer, these community types differed significantly in the winter. (iii) In the summer, most bay samples had a common 121-nucleotide 5S rRNA molecule. Although this band occurred in the top water of midbay samples, it did not occur in particle-associate...Continue Reading

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Citations

Aug 14, 1998·Environmental Health Perspectives·T FordJ Shine
Jan 5, 2002·Applied and Environmental Microbiology·Hidetoshi UrakawaDavid A Stahl
May 7, 2003·Applied and Environmental Microbiology·Hidetoshi UrakawaDavid A Stahl
Sep 11, 2007·Applied and Environmental Microbiology·Jinjun KanFeng Chen
Sep 25, 2014·Frontiers in Microbiology·Holly M SimonLydie Herfort
Nov 7, 2006·Applied and Environmental Microbiology·Christian WinterAndreas H Farnleitner
Jan 7, 2003·Applied and Environmental Microbiology·Richard A LongFarooq Azam
Dec 8, 2005·Applied and Environmental Microbiology·Alex PozhitkovPeter A Noble
Dec 25, 2015·Microbiology and Molecular Biology Reviews : MMBR·Hongyue Dang, Charles R Lovell
May 17, 2005·Environmental Microbiology·Christian WinterMarkus G Weinbauer
Feb 7, 2002·Journal of Microbiological Methods·Eugenio Parente, Annamaria Ricciardi

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