Natural Mutagenesis-Enabled Global Proteomic Study of Metabolic and Carbon Source Implications in Mutant Thermoacidophillic Archaeon Sulfolobus solfataricus PBL2025

Journal of Proteome Research
Wen QiuPhillip C Wright

Abstract

The thermoacidophilic crenarchaeon Sulfolobus solfataricus has been widely used as a model organism for archaeal systems biology research. Investigation using its spontaneous mutant PBL2025 provides an effective metabolic baseline to study subsequent mutagenesis-induced functional process shifts as well as changes in feedback inhibitions. Here, an untargeted metabolic investigation using quantitative proteomics and metabolomics was performed to correlate changes in S. solfataricus strains P2 against PBL2025 and under both glucose and tryptone. The study is combined with pathway enrichment analysis to identify prominent proteins with differential stoichiometry. Proteome level quantification reveals that over 20% of the observed overlapping proteome is differentially expressed under these conditions. Metabolic-induced differential expressions are observed along the central carbon metabolism, along with 12 other significantly regulated pathways. Current findings suggest that PBL2025 is able to compensate through the induction of carbon metabolism, as well as other anabolic pathways such as Val, Leu and iso-Leu biosynthesis. Studying protein abundance changes after changes in carbon sources also reveals distinct differences in meta...Continue Reading

References

Jan 1, 1978·Annual Review of Biochemistry·H E Umbarger
Mar 14, 1991·European Journal of Biochemistry·V ConsalviR Scandurra
Apr 1, 1990·Molecular & General Genetics : MGG·A M SanangelantoniO Tiboni
Dec 10, 1998·Nucleic Acids Research·H OgataM Kanehisa
Jun 12, 1999·European Journal of Clinical Nutrition·J T Brosnan
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·T KnöchelJ N Jansonius
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·Q SheJ Van der Oost
Jun 28, 2002·The Journal of Biological Chemistry·Oliver E OwenRichard W Hanson
Nov 27, 2002·Antonie van Leeuwenhoek·Wil N KoningsArnold J M Driessen
May 29, 2003·The Journal of Nutrition·John T Brosnan
Dec 4, 2003·Biochemical Society Transactions·P R Rich
May 14, 2004·Journal of Applied Microbiology·F Diez-Gonzalez, Y Karaibrahimoglu
Jun 1, 2004·Journal of Bioenergetics and Biomembranes·Sonja-Verena AlbersArnold J Driessen
May 5, 2005·The Biochemical Journal·Hatim AhmedBettina Siebers
Oct 20, 2005·Journal of Bioscience and Bioengineering·C B Park, S B Lee
Dec 21, 2005·The Journal of Nutrition·John T Brosnan, Margaret E Brosnan
Feb 24, 2007·Journal of Proteome Research·Poh Kuan ChongPhillip C Wright
Jun 11, 2008·Archives of Microbiology·Silvia Berkner, Georg Lipps
Sep 24, 2008·Journal of Proteome Research·Trong Khoa Pham, Phillip C Wright
Sep 17, 2009·Journal of Proteome Research·Saw Yen OwPhillip C Wright
Oct 6, 2009·Extremophiles : Life Under Extreme Conditions·Melanie ZapartyBettina Siebers
Oct 27, 2009·Journal of Bacteriology·Behnam ZolghadrSonja-Verena Albers
Oct 30, 2009·International Journal of Food Microbiology·Avelino Alvarez-OrdóñezMercedes López
Dec 4, 2009·Journal of Proteome Research·Trong Khoa PhamPhillip C Wright
Apr 13, 2010·Journal of Bacteriology·M GogliettinoG Palmieri
Jan 5, 2011·BMC Systems Biology·Atsushi FukushimaKazuki Saito
Jan 27, 2011·Biochemical Society Transactions·Patrick HaferkampBettina Siebers
Dec 28, 2011·Journal of Experimental Botany·Arren Bar-EvenRon Milo
Aug 29, 2012·Journal of Applied Microbiology·C Feehily, K A G Karatzas
Sep 4, 2012·Applied and Environmental Microbiology·Sreedevi LalithambikaPaul Blum
Oct 26, 2012·BMC Bioinformatics·Irene Sánchez-LinaresJosé M García

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.