Natural Product Glycosylation: Biocatalytic Synthesis of Quercetin-3,4'-O-diglucoside

Applied Biochemistry and Biotechnology
Ping SunKequan Chen

Abstract

Flavonoids have gained much attention for their proposed positive effects for human health. Glycosylation is a significant method for the structural modification of various flavanols, resulting in glycosides with increased solubility, stability, and bioavailability compared with the corresponding aglycone. Natural product glycosylation by using enzymes has emerged as a topic of interest as it offers a sustainable and economical alternative source so as to address supply scalability limitations associated with plant-based production. Quercetin-3,4'-O-diglucoside, as one of the major but trace bioactive flavonoids in onion (Allium cepa), is superior or at least equal to quercetin aglycone in its bioavailability. In the present study, the onion-derived enzyme, UGT73G1, coupled with sucrose synthase, StSUS1, from Solanum tuberosum formed a circulatory system to produce quercetin-3,4'-O-diglucoside from quercetin, which preferred sucrose as a sugar donor and quercetin as a sugar acceptor. The optimal conditions were determined in order to increase the production of quercetin-3,4'-O-diglucoside. The maximum concentration of quercetin-3,4'-O-diglucoside achieved in a 10-mL reaction was 427.11 mg/L, from the conversion of 1 g/L of quer...Continue Reading

References

Sep 28, 1999·Trends in Plant Science·R A Dixon, C L Steele
Oct 22, 2003·Phytochemistry·Catherine M KramerGerson Graser
Jan 11, 2005·The American Journal of Clinical Nutrition·Barry HalliwellAndrew Jenner
Jun 25, 2005·Chembiochem : a European Journal of Chemical Biology·Lothar EllingWolfgang Piepersberg
Oct 10, 2006·Journal of Bioscience and Bioengineering·Ji Hye KimJoong-Hoon Ahn
Feb 27, 2007·The Journal of Nutritional Biochemistry·Sonia Ramos
Jun 24, 2008·Applied Microbiology and Biotechnology·Xian-Zhi HeRichard A Dixon
Nov 14, 2008·Nutrition and Cancer·Haitao LuoYi Charlie Chen
Nov 15, 2008·Journal of Cellular Biochemistry·Jae-Hoon JeongYong J Lee
Sep 10, 2010·Antiviral Research·Yunjeong KimKyeong-Ok Chang
May 2, 2014·Biotechnology Advances·Jianbo XiaoMilen I Georgiev
Aug 13, 2015·Bioscience, Biotechnology, and Biochemistry·Yu WangLin Xu
Sep 4, 2015·Chemical Society Reviews·Dong-Mei LiangJian-Jun Qiao
Sep 17, 2015·Journal of Food Science·Yasuaki KashinoJunji Terao
Dec 15, 2015·Biotechnology Advances·Katharina SchmölzerBernd Nidetzky

❮ Previous
Next ❯

Citations

Oct 15, 2021·Journal of Asian Natural Products Research·Jia-Nan CaoPing Song

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.