Natural pyrethrins induce autophagy of HepG2 cells through the activation of AMPK/mTOR pathway

Environmental Pollution
Yun YangLiming Tao

Abstract

Natural pyrethrins, one kind of insects' neural toxin, have been used worldwide for the control of pests of crops, livestock, and human beings. However, their specific mechanisms of action are incompletely understood and hence further investigation is required. Here we used a series of experiments including colony formation, fluorescent staining, western blotting, enzyme activity detection, immunofluorescence analysis, and real-time quantitative PCR (QPCR) to investigate whether natural pyrethrins (0-40 μg/mL) are able to modulate autophagy process through AMPK/mTOR signaling pathway, in order to reveal their cytotoxic mechanisms. The results showed that natural pyrethrins markedly inhibited the proliferation of HepG2 cells in both concentration- and time-dependent manners. Particularly, natural pyrethrins could induce the resulting autophagosome, and the intensification of LC3-II formation and translocation, the accumulation of Beclin-1 and the reduction of p62 and thus autophagy. We clarified that natural pyrethrins induced the abnormal level of oxidation reduction metabolism, leading to mitochondrial permeability transition pore (mPTP) opening, ATP depletion and mitochondria eliminating by autophagy. Moreover, the phosphoryl...Continue Reading

Citations

Oct 4, 2020·Ecotoxicology and Environmental Safety·Jianzhao LiaoZhaoxin Tang
Jun 21, 2020·The Science of the Total Environment·Yun YangLiming Tao
Aug 6, 2021·Ecotoxicology and Environmental Safety·Heng YinYanqiu Zhu
Sep 24, 2019·Journal of Agricultural and Food Chemistry·Youwu HaoLiming Tao
Jan 7, 2022·Annual Review of Pharmacology and Toxicology·Xiaojing WangMaría-Aránzazu Martínez

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