Natural resistance to infection with intracellular pathogens: cross-talk between Nramp1 and Lps genes

Electrophoresis
H KovárováA Macela

Abstract

This study was designed to analyze the association of Nramp1 and/or Lps genes with differential protein expression in macrophages in order to select candidate proteins that might be related to resistance/susceptibility to various microbial infections under the control of the Nramp1 and/or Lps genes. The macrophage cell lines derived from bone marrow of Nramp1 or Lps congenic mice were utilized and high-resolution two-dimensional electrophoreis (2-DE), separating proteins according to their charge and size, was used as a window into alterations in gene expression and a means to compare the macrophages carrying a resistant allele of Nramp1 gene and/or normal allele of Lps gene, with their counterparts carrying either a susceptible allele of Nramp1 or defective allele of the Lps gene. We demonstrate that the changes of constitutive levels of two proteins named according to their isoelectric point/molecular weight (pI/Mr), p6.6/25 and p7.0/22, discriminate satisfactorily not only the macrophages congenic at the Nramp1 gene but also the macrophages congenic at the Lps gene, thus reflecting their common genotype (Nramp1r, Lps[n]). Furthermore, the decreased constitutive levels of these proteins in macrophages carrying a defective all...Continue Reading

References

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Citations

Jul 20, 2002·Veterinary Immunology and Immunopathology·P M van DiemenT S Wallis
Jun 14, 2006·Journal of Mass Spectrometry : JMS·Alexandr JegorovVladimir Havlicek

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